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Quantitative affinity measurement of small molecule ligand binding to major histocompatibility complex class-I-related protein 1 MR1
- Source :
- The Journal of biological chemistry. 298(12)
- Publication Year :
- 2022
-
Abstract
- The Major Histocompatibility Complex class I-related protein 1 (MR1) presents small molecule metabolites, drugs, and drug-like molecules that are recognized by MR1-reactive T cells. While we have an understanding of how antigens bind to MR1 and upregulate MR1 cell surface expression, a quantitative, cell-free, assessment of MR1 ligand-binding affinity was lacking. Here, we developed a fluorescence polarization-based assay in which fluorescent MR1 ligand was loaded into MR1 protein in vitro and competitively displaced by candidate ligands over a range of concentrations. Using this assay, ligand affinity for MR1 could be differentiated as strong (IC
Details
- ISSN :
- 1083351X
- Volume :
- 298
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- The Journal of biological chemistry
- Accession number :
- edsair.doi.dedup.....754fd3adf15fa71a5de6aa8600ff8e61