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PML protein analysis using imaging flow cytometry

Authors :
Emma Gudgin
Lizz F. Grimwade
David Bloxham
Michael A. Scott
Wendy N. Erber
Source :
Journal of Clinical Pathology. 64:447-450
Publication Year :
2010
Publisher :
BMJ, 2010.

Abstract

Acute promyelocytic leukaemia (APML) can be promptly diagnosed by detecting abnormal diffuse staining patterns of PML bodies in abnormal promyelocytes using immunofluorescence microscopy. However, this technique is subjective, with low sensitivity. Using samples from 18 patients with acute myeloid leukaemia (AML) (including four with APML), the authors investigated whether imaging flow cytometry could be a viable alternative to this current technique and improve sensitivity levels. Bone marrow/peripheral blood cells were stained with an antibody to PML, and data were acquired on an ImageStream (Amnis Corporation, Seattle, Washington, USA). Using the modulation feature for data analysis, the authors demonstrated that this technique could successfully identify cases of APML. Imaging flow cytometry, by analysing greater numbers of cells and with the potential to include disease-specific antigens, increases the sensitivity of the current immunofluorescence technique. Imaging flow cytometry is an exciting technology that has many possible applications in the diagnosis of haematological malignancies, including the potential to integrate modalities.

Details

ISSN :
14724146 and 00219746
Volume :
64
Database :
OpenAIRE
Journal :
Journal of Clinical Pathology
Accession number :
edsair.doi.dedup.....754af8878d0bbde2f60e261acf5b1075