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Usefulness of the ElliPro epitope predictor program in defining the repertoire of HLA-ABC eplets
- Source :
- Human Immunology. 78:481-488
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- HLA matching at the epitope level offers new opportunities to identify suitable donors for transplant patients. The International HLA Epitope Registry (www.Epregistry.com.br) describes for the various HLA loci, repertoires of eplets including those that correspond to epitopes experimentally verified with specific antibodies. There are also many eplets which have remained as theoretical entities because no informative antibodies have been found. Which of them have immunogenic potential or conversely, might be considered as non-epitopes that cannot elicit specific antibody responses? This question is important for the application of epitope-based HLA matching in clinical transplantation. Correct predictions of B-cell epitopes on antigenic proteins are essential to the effective design of microbial vaccines and the development of specific antibodies used in immunotherapy and immunodiagnostics but prediction programs based on structural and physiochemical properties of amino acid residues are generally ineffective. Recent prediction programs based on three-dimensional structures of antigen-antibody complexes are more promising. One such program is called ElliPro developed by Ponomarenko. This report describes studies demonstrating that ElliPro can predict alloantibody responses to HLA-ABC eplets. Antibody-verified eplets have amino acid residues with much higher ElliPro scores than eplets for which no specific antibodies have been found. The latter group includes residues with very low ElliPro scores; they appear to represent eplets that might be classified as non-epitopes. In conclusion, ElliPro offers a new approach to characterize epitope repertoires that are clinically relevant in HLA matching.
- Subjects :
- 0301 basic medicine
Protein Conformation
Immunology
Antigen-Antibody Complex
HLA-C Antigens
Human leukocyte antigen
Computational biology
Histocompatibility Testing
Immunologic Tests
Biology
Epitope
Isoantibodies
03 medical and health sciences
0302 clinical medicine
Antigen
Transplantation Immunology
HLA-B Antigens
Humans
Immunology and Allergy
Registries
Vaccines
Immunodiagnostics
HLA-A Antigens
Computational Biology
General Medicine
Transplantation
030104 developmental biology
Haplotypes
Epitopes, B-Lymphocyte
Immunotherapy
Software
030215 immunology
Subjects
Details
- ISSN :
- 01988859
- Volume :
- 78
- Database :
- OpenAIRE
- Journal :
- Human Immunology
- Accession number :
- edsair.doi.dedup.....754a74ad50185e131b8a745c34880de2