Somatic embryogenesis and plant regeneration in elite clones of Theobroma cacao

Authors :: Luciana C. Cidade
Thiago Édson Ribeiro da Silva
J.C.M. Cascardo
Marcio Gilberto Cardoso Costa
Fatima Cerqueira Alvim
Source :: Pesquisa Agropecuária Brasileira v.43 n.10 2008, Pesquisa Agropecuária Brasileira, Empresa Brasileira de Pesquisa Agropecuária (Embrapa), instacron:EMBRAPA
Publication Year :: 2008
Publisher :: Embrapa Secretaria de Pesquisa e Desenvolvimento, 2008.
Abstract: The objective of this work was to evaluated a procedure for somatic embryogenesis and regeneration of cacao (Theobroma cacao L.) elite clones. Petal explants from cacao clones TSH 565 and TSH 1188 were cultured on PCG and SCG-2 media, for calli growth. Somatic embryos were formed on the surface of embryogenic calli after transfer to embryo development (ED) medium. Clone TSH 565 showed a higher embryogenic potential than TSH 1188. The best combination of carbon source for embryo induction in ED medium was genotype-specific. Embryogenic callus formations increased in micropore tape-sealed Petri dishes, irrespective of cacao genotype. Mature somatic embryos were successfully converted into plantlets.

Language :: English
Database :: OpenAIRE
Journal :: Pesquisa Agropecuária Brasileira v.43 n.10 2008, Pesquisa Agropecuária Brasileira, Empresa Brasileira de Pesquisa Agropecuária (Embrapa), instacron:EMBRAPA
Accession number :: edsair.doi.dedup.....7549034ecf7ea440936439e7147a0ae3

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