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Analysis of the role of LinA and LinB in biodegradation of ?-hexachlorocyclohexane
- Source :
- Environmental Microbiology. 9:2331-2340
- Publication Year :
- 2007
- Publisher :
- Wiley, 2007.
-
Abstract
- Commercial formulations of hexachlorocyclohexane (HCH) consist of a mixture of four isomers, alpha, beta, gamma and delta. All these four isomers are toxic and recalcitrant pollutants. Sphingobium (formerly Sphingomonas) sp. strain BHC-A is able to degrade all four HCH isomers. Eight lin genes responsible for the degradation of gamma-HCH in BHC-A were cloned and analysed for their role in the degradation of delta-HCH, and the initial conversion steps in delta-HCH catabolism by LinA and LinB in BHC-A were found. LinA dehydrochlorinated delta-HCH to produce 1,3,4,6-tetrachloro-1,4-cyclohexadiene (1,4-TCDN) via delta-pentachlorocyclohexene (delta-PCCH). Subsequently, both 1,4-TCDN and delta-PCCH are catalysed by LinB via two successive rounds of hydrolytic dechlorinations to form 2,5-dichloro-2,5-cyclohexadiene-1,4-diol (2,5-DDOL) and 2,3,5-trichloro-5-cyclohexene-1,4-diol (2,3,5-TCDL) respectively. LinB could also catalyse the hydrolytic dechlorination of delta-HCH to 2,3,5,6-tetrachloro-1,4-cyclohexanediol (TDOL) via 2,3,4,5,6-pentachlorocyclohexanol (PCHL).
- Subjects :
- Delta-hexachlorocyclohexane
biology
Stereochemistry
Catabolism
Molecular Sequence Data
Hexachlorocyclohexane
Lyases
Biodegradation
biology.organism_classification
Sphingomonas
Microbiology
Sphingobium
Hydrolysis
chemistry.chemical_compound
Biodegradation, Environmental
chemistry
Metabolic Networks and Pathways
Ecology, Evolution, Behavior and Systematics
Subjects
Details
- ISSN :
- 14622920 and 14622912
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Environmental Microbiology
- Accession number :
- edsair.doi.dedup.....7545d55a699640adb2060f32309464d7