Confined electrochemiluminescence imaging microarray for high-throughput biosensing of single cell-released dopamine

The quantitative detection of single cell secretions is always limited by their accurate collection and the heterogeneity of different cells. In this work, a confined electrochemiluminescence (ECL) imaging microarray (CEIM) chip was designed to capture single or a few cells in each cylindrical microwell for high-throughput quantitation of cell-secreted dopamine (DA). The ITO surface at the bottom of microwells was functionalized with the film of DA aptamer modified coreactant-embedded polymer dots (Pdots), which endowed the chip with the abilities to both in situ recognize the target DA secreted from the cells and emit the ECL signal for responding the secreted target without need of any additional coreactant. At the applied potential of +1.4 V, the Pdots in the film emitted strong ECL signal, which could be quenched by the electrochemical oxidation product of DA in individual microwell for sensitive detection of single cell-released DA. The practicability of the proposed CEIM chip along with the ECL imaging and biosensing strategy was demonstrated by evaluating the amounts of single cell-released DA in different microwells under hypoxia stimulation. This protocol revealed the heterogeneity of cell secretion, and could be extended for quantitation of other secretions from different kinds of single cells.