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Combined Use of Morphological and Molecular Tools to Resolve Species Mis-Identifications in the Bivalvia The Case of Glycymeris glycymeris and G. pilosa
- Source :
- PLoS ONE, Plos One (1932-6203) (Public Library Science), 2016-09, Vol. 11, N. 9, P. e0162059. (21p.), PLoS ONE, Vol 11, Iss 9, p e0162059 (2016), PLoS ONE, Public Library of Science, 2016, 11 (9), pp.e0162059. ⟨10.1371/journal.pone.0162059⟩, PLoS ONE, 2016, 11 (9), pp.e0162059. ⟨10.1371/journal.pone.0162059⟩
- Publication Year :
- 2016
- Publisher :
- Public Library of Science, 2016.
-
Abstract
- 00000 ăWOS:000384167300004; International audience; Morphological and molecular tools were combined to resolve the misidentification between Glycymeris glycymeris and Glycymeris pilosa from Atlantic and Mediterranean populations. The ambiguous literature on the taxonomic status of these species requires this confirmation as a baseline to studies on their ecology and sclerochronology. We used classical and landmark-based morphometric approaches and performed bivariate and multivariate analyses to test for shell character interactions at the individual and population level. Both approaches generated complementary information. The former showed the shell width to length ratio and the valve asymmetry to be the main discriminant characters between Atlantic and Mediterranean populations. Additionally, the external microsculpture of additional and finer secondary ribs in G. glycymeris discriminates it from G. pilosa. Likewise, landmark-based geometric morphometrics revealed a stronger opisthogyrate beak and prosodetic ligament in G. pilosa than G. glycymeris. Our Bayesian and maximum likelihood phylogenetic analyses based on COI and ITS2 genes identified that G. glycymeris and G. pilosa form two separate monophyletic clades with mean interspecific divergence of 11% and 0.9% for COI and ITS2, respectively. The congruent patterns of morphometric analysis together with mitochondrial and nuclear phylogenetic reconstructions indicated the separation of the two coexisting species. The intraspecific divergence occurred during the Eocene and accelerated during the late Pliocene and Pleistocene. Glycymeris pilosa showed a high level of genetic diversity, appearing as a more robust species whose tolerance of environmental conditions allowed its expansion throughout the Mediterranean.
- Subjects :
- 0301 basic medicine
Pilosa
lcsh:Medicine
Monophyly
Sclerochronology
Medicine and Health Sciences
mollusca-bivalvia
lcsh:Science
Data Management
Multidisciplinary
biology
Phylogenetic tree
Ecology
dna polymorphism
Phylogenetic Analysis
Phylogenetics
Connective Tissue
Anatomy
section
Research Article
Bivalves
Computer and Information Sciences
sea
Imaging Techniques
growth
dog cockle
Research and Analysis Methods
03 medical and health sciences
sclerochronological archive
Genetics
Animals
patterns
Evolutionary Systematics
14. Life underwater
bivalve
identification
Glycymeris
Adriatic
phylogenetics
Molecular Biology Techniques
Molecular Biology
Taxonomy
Morphometrics
Genetic diversity
Evolutionary Biology
Molecular Biology Assays and Analysis Techniques
Ligaments
Population Biology
ACL
Morphometry
lcsh:R
Ecology and Environmental Sciences
Organisms
Biology and Life Sciences
Paleontology
Molluscs
biology.organism_classification
Invertebrates
030104 developmental biology
Biological Tissue
Haplotypes
Evolutionary biology
Earth Sciences
arctica-islandica
lcsh:Q
Paleoecology
[SDE.BE]Environmental Sciences/Biodiversity and Ecology
Paleobiology
Paleogenetics
Dog cockle
Population Genetics
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 11
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....751d36ea4e31ba4847bf57cd73258c63