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Improved stability of recombinant hemagglutinin using a formulation containing sodium thioglycolate
- Source :
- Vaccine. 33(44)
- Publication Year :
- 2015
-
Abstract
- This study was designed to improve the stability of liquid formulations of recombinant influenza hemagglutinin (rHA) and to understand the mechanism of early loss of potency for rHA. The potency of rHA derived from several influenza strains was determined using single radial immunodiffusion (SRID), and the structure of the rHA was characterized using SDS-PAGE and dynamic light scattering. rHA formed disulfide cross-linked multimers, and potency decreased during extended storage. To reduce disulfide-mediated cross-linking and early potency loss, rHA was formulated with sodium thioglycolate (STG) and citrate. Addition of 80 mM STG and 55 mM sodium citrate inhibited disulfide-mediated cross-linking without affecting protein function for each rHA tested. The shelf life of the rHA formulation with STG-citrate, based on potency as determined by SRID, was extended as much as 20-fold, compared to a control formulation without STG-citrate. STG-citrate did not have a significant effect on the immunogenicity of H1 A/California/7/2009 rHA in mice.
- Subjects :
- Immunodiffusion
Antibodies, Viral
law.invention
chemistry.chemical_compound
law
Immunology and Microbiology(all)
Sodium citrate
Potency
Animals
Polyacrylamide gel electrophoresis
Vaccine Potency
Radial immunodiffusion
Mice, Inbred BALB C
Chromatography
General Veterinary
General Immunology and Microbiology
Immunogenicity
Public Health, Environmental and Occupational Health
Hemagglutinin
veterinary(all)
SODIUM THIOGLYCOLATE
Dynamic Light Scattering
Recombinant Proteins
Infectious Diseases
Hemagglutinins
chemistry
Biochemistry
Influenza Vaccines
Thioglycolates
Recombinant DNA
Molecular Medicine
Electrophoresis, Polyacrylamide Gel
Subjects
Details
- ISSN :
- 18732518
- Volume :
- 33
- Issue :
- 44
- Database :
- OpenAIRE
- Journal :
- Vaccine
- Accession number :
- edsair.doi.dedup.....751afe3527d89275cf0dea344e293ad2