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A plasmid involved in chloramphenicol production in Streptomyces venezuelae: evidence from genetic mapping
- Source :
- Journal of general microbiology. 90(2)
- Publication Year :
- 1975
-
Abstract
- SUMMARY: To test the hypothesis that chloramphenicol production in Streptomyces venezuelae depends on the presence of a plasmid, mapping analysis was carried out by using eight markers in addition to chloramphenicol production and melanoid pigment formation. The sequence of the eight markers was determined on a circular linkage map as follows: -his-ade-str-leu-lys-met-ilv-pro-(his-). This sequence resulted in the frequency of quadruple crossover (q.c.o.) recombinants having the lowest value, 3·2 to 4·9%. However, the character of chloramphenicol non-production, which was obtained by incubating mycelia with acriflavin, was not located on this linkage map; more than 15% q.c.o. recombinants would have been required to explain the results. From these results and other tests, it is concluded that chloramphenicol production is controlled by a plasmid. This plasmid appeared to be non-transferable in conjugation.
- Subjects :
- Streptomyces venezuelae
Genetics
Recombination, Genetic
biology
Genetic Linkage
Chloramphenicol
Extrachromosomal Inheritance
Chromosome Mapping
Pigments, Biological
biology.organism_classification
Microbiology
Molecular biology
Streptomyces
Plasmid
Gene mapping
Genetic linkage
medicine
Acriflavine
Pigment formation
Mycelium
Crosses, Genetic
medicine.drug
Plasmids
Subjects
Details
- ISSN :
- 00221287
- Volume :
- 90
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Journal of general microbiology
- Accession number :
- edsair.doi.dedup.....7515dac27ebcff25aa51ea40ccd2f536