Back to Search Start Over

A plasmid involved in chloramphenicol production in Streptomyces venezuelae: evidence from genetic mapping

Authors :
M. Okanishi
Hisayoshi Akagawa
H. Umezawa
Source :
Journal of general microbiology. 90(2)
Publication Year :
1975

Abstract

SUMMARY: To test the hypothesis that chloramphenicol production in Streptomyces venezuelae depends on the presence of a plasmid, mapping analysis was carried out by using eight markers in addition to chloramphenicol production and melanoid pigment formation. The sequence of the eight markers was determined on a circular linkage map as follows: -his-ade-str-leu-lys-met-ilv-pro-(his-). This sequence resulted in the frequency of quadruple crossover (q.c.o.) recombinants having the lowest value, 3·2 to 4·9%. However, the character of chloramphenicol non-production, which was obtained by incubating mycelia with acriflavin, was not located on this linkage map; more than 15% q.c.o. recombinants would have been required to explain the results. From these results and other tests, it is concluded that chloramphenicol production is controlled by a plasmid. This plasmid appeared to be non-transferable in conjugation.

Details

ISSN :
00221287
Volume :
90
Issue :
2
Database :
OpenAIRE
Journal :
Journal of general microbiology
Accession number :
edsair.doi.dedup.....7515dac27ebcff25aa51ea40ccd2f536