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Hemagglutinin mutation D222N of the 2009 pandemic H1N1 influenza virus alters receptor specificity without affecting virulence in mice
- Source :
- Virus research. 189
- Publication Year :
- 2013
-
Abstract
- The D222N hemagglutinin (HA) mutation within the receptor-binding site was detected with higher frequencies in severe cases of 2009 pandemic H1N1 (pdmH1N1) infections. The impact of this mutation was investigated in vitro and in vivo using recombinant viruses. The recombinant D222N virus grew to significantly lower viral titers than the WT in A549 but not in MDCK cells. A dose-dependent glycan array analysis with the D222N virus showed a modest increase in the binding avidity to human-like (α-2,6 sialylated glycan) receptors and avian-like (α-2,3 sialylated glycan) receptors than the WT virus. The D222N HA mutation resulted in slight weight loss, lower lung titers, inflammatory cytokines and alveolar inflammation in mice than the WT virus. This may or may not be associated with severe clinical outcomes reported in humans.
- Subjects :
- Cancer Research
Glycan
Mutation, Missense
Virulence
Hemagglutinin (influenza)
Virus Attachment
Hemagglutinin Glycoproteins, Influenza Virus
Biology
medicine.disease_cause
Virus
law.invention
Cell Line
Influenza A Virus, H1N1 Subtype
Orthomyxoviridae Infections
law
Virology
medicine
Animals
Humans
Avidity
Receptor
Lung
Inflammation
Mutation
Mice, Inbred BALB C
Body Weight
Viral Load
Disease Models, Animal
Infectious Diseases
Amino Acid Substitution
biology.protein
Recombinant DNA
Cytokines
Female
Mutant Proteins
Subjects
Details
- ISSN :
- 18727492
- Volume :
- 189
- Database :
- OpenAIRE
- Journal :
- Virus research
- Accession number :
- edsair.doi.dedup.....75030ed2459faaf03d99a69fa66e1f62