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Long-Term, Noninvasive In Vivo Tracking of Progenitor Cells Using Multimodality Photoacoustic, Optical Coherence Tomography, and Fluorescence Imaging
- Source :
- ACS Nano
- Publication Year :
- 2021
- Publisher :
- American Chemical Society (ACS), 2021.
-
Abstract
- Stem cell regenerative medicine therapies have emerged as promising treatments for currently incurable diseases. A remaining challenge for cell therapies is the ability to track the migration and distribution of the transplanted cells in a long-term, noninvasive manner in vivo to assess their efficacy. This study develops a noninvasive, and high spatial resolution photoacoustic microscopy (PAM) and optical coherence tomography (OCT) imaging system for in vivo tracking of subretinally injected progenitor human retinal pigment epithelium cells (ARPE-19) labeled with chainlike gold nanoparticle (CGNP) clusters in RPE damage. CGNP provided significant PAM, OCT, and fluorescence signals to selectively track the migration of ARPE-19 cells in living rabbit eyes for 3 months. PAM and OCT imaging allow accurate anatomical information to determine the exact retinal layer in which the transplanted ARPE-19 cells are located which was confirmed by histology. This presents an efficient and advanced technology to visualize fundamental biological processes of cell therapies in complex in vivo environments in real time.
- Subjects :
- Fluorescence-lifetime imaging microscopy
Metal Nanoparticles
General Physics and Astronomy
Regenerative medicine
Article
Photoacoustic Techniques
chemistry.chemical_compound
Optical coherence tomography
In vivo
medicine
Animals
Humans
General Materials Science
Progenitor cell
Retinal pigment epithelium
medicine.diagnostic_test
business.industry
Stem Cells
Optical Imaging
General Engineering
Retinal
medicine.anatomical_structure
chemistry
Rabbits
Gold
Stem cell
business
Tomography, Optical Coherence
Biomedical engineering
Subjects
Details
- ISSN :
- 1936086X and 19360851
- Volume :
- 15
- Database :
- OpenAIRE
- Journal :
- ACS Nano
- Accession number :
- edsair.doi.dedup.....74f92708f211aec4c9ec68bea6be2679