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Micro RNA-125b (miRNA-125b) function in astrogliosis and glial cell proliferation
- Source :
- Neuroscience letters. 476(1)
- Publication Year :
- 2010
-
Abstract
- Micro RNAs (miRNAs) are post-transcriptional modulators of gene expression that regulate the stability and translation of their target messenger RNAs (mRNAs). Here we report that the levels of a human brain-enriched miRNA-125b are up-regulated in interleukin-6 (IL-6)-stressed normal human astrocytes (NHA), a treatment known to induce astrogliosis. In vitro, anti-miRNA-125b added exogenously to IL-6-stressed NHA cultures attenuated both glial cell proliferation and increased the expression of the cyclin-dependent kinase inhibitor 2A (CDKN2A), a miRNA-125b target and negative regulator of cell growth. A strong positive correlation between miRNA-125b abundance and the glial cell markers glial fibrillary acidic protein (GFAP) and vimentin, and CDKN2A down-regulation was noted in advanced Alzheimer's disease (AD) and in Down's syndrome (DS) brain, chronic neurological disorders associated with astrogliosis. The results suggest that miRNA-125b up-regulation contributes to astrogliosis and to defects in the cell cycle that are characteristic of degenerating brain tissues.
- Subjects :
- Glial fibrillary acidic protein
Cell growth
Interleukin-6
General Neuroscience
Cell cycle
Biology
medicine.disease
GFAP stain
Glial cell proliferation
Actins
Astrogliosis
Cell biology
Up-Regulation
MicroRNAs
Astrocytes
Gene expression
microRNA
Glial Fibrillary Acidic Protein
medicine
biology.protein
Humans
Vimentin
Cells, Cultured
Cyclin-Dependent Kinase Inhibitor p16
Cell Proliferation
Subjects
Details
- ISSN :
- 18727972
- Volume :
- 476
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Neuroscience letters
- Accession number :
- edsair.doi.dedup.....74f4ffd78f7fff93d5464fe0decad547