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Induced pluripotent stem cells-derived myeloid-derived suppressor cells regulate the CD8+ T cell response

Authors :
Masayuki Fujino
Ryoko Araki
Xiao-Kang Li
Lina Lu
Daniel Joyce
John J. Fung
Shiguang Qian
Miwa Morita
Source :
Stem Cell Research, Vol 29, Iss, Pp 32-41 (2018)
Publication Year :
2018
Publisher :
Elsevier BV, 2018.

Abstract

Myeloid-derived suppressor cells (MDSCs) are markedly increased in cancer patients and tumor-bearing mice and promote tumor growth and survival by inhibiting host innate and adaptive immunity. In this study, we generated and characterized MDSCs from murine-induced pluripotent stem cells (iPSCs). The iPSCs were co-cultured with OP9 cells, stimulated with GM-CSF, and became morphologically heterologous under co-culturing with hepatic stellate cells. Allogeneic and OVA-specific antigen stimulation demonstrated that iPS-MDSCs have a T-cell regulatory function. Furthermore, a popliteal lymph node assay and autoimmune hepatitis model showed that iPS-MDSCs also regulate immune responsiveness in vivo and have a therapeutic effect against hepatitis. Taken together, our results demonstrated a method of generating functional MDSCs from iPSCs and highlighted the potential of iPS-MDSCs as a key cell therapy resource for transplantation and autoimmune diseases. Keywords: Myeloid-derived suppressor cells, Induced pluripotent stem cells, T cell response

Details

ISSN :
18735061
Volume :
29
Database :
OpenAIRE
Journal :
Stem Cell Research
Accession number :
edsair.doi.dedup.....74e5d3cf6f6c9443b4b4ad0a3c9b30b8
Full Text :
https://doi.org/10.1016/j.scr.2018.03.009