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Induced pluripotent stem cells-derived myeloid-derived suppressor cells regulate the CD8+ T cell response
- Source :
- Stem Cell Research, Vol 29, Iss, Pp 32-41 (2018)
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- Myeloid-derived suppressor cells (MDSCs) are markedly increased in cancer patients and tumor-bearing mice and promote tumor growth and survival by inhibiting host innate and adaptive immunity. In this study, we generated and characterized MDSCs from murine-induced pluripotent stem cells (iPSCs). The iPSCs were co-cultured with OP9 cells, stimulated with GM-CSF, and became morphologically heterologous under co-culturing with hepatic stellate cells. Allogeneic and OVA-specific antigen stimulation demonstrated that iPS-MDSCs have a T-cell regulatory function. Furthermore, a popliteal lymph node assay and autoimmune hepatitis model showed that iPS-MDSCs also regulate immune responsiveness in vivo and have a therapeutic effect against hepatitis. Taken together, our results demonstrated a method of generating functional MDSCs from iPSCs and highlighted the potential of iPS-MDSCs as a key cell therapy resource for transplantation and autoimmune diseases. Keywords: Myeloid-derived suppressor cells, Induced pluripotent stem cells, T cell response
- Subjects :
- 0301 basic medicine
Cell Biology
General Medicine
Biology
Acquired immune system
Transplantation
Cell therapy
03 medical and health sciences
030104 developmental biology
Immune system
lcsh:Biology (General)
Myeloid-derived Suppressor Cell
Hepatic stellate cell
Cancer research
Cytotoxic T cell
Induced pluripotent stem cell
lcsh:QH301-705.5
Developmental Biology
Subjects
Details
- ISSN :
- 18735061
- Volume :
- 29
- Database :
- OpenAIRE
- Journal :
- Stem Cell Research
- Accession number :
- edsair.doi.dedup.....74e5d3cf6f6c9443b4b4ad0a3c9b30b8
- Full Text :
- https://doi.org/10.1016/j.scr.2018.03.009