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A high activity zinc transporter OsZIP9 mediates zinc uptake in rice

Authors :
Limin Liang
Jingjing Tian
Yutong Li
Guangyuan Wang
Zonghao Liu
Meng Yang
Fangsen Xu
Xingming Lian
Qingqing Du
Deng Cheng
Yu Qiu
Hongmei Cai
Lei Shi
Source :
The Plant Journal. 103:1695-1709
Publication Year :
2020
Publisher :
Wiley, 2020.

Abstract

Zinc (Zn) is an essential micronutrient for most organisms including humans, and Zn deficiency is widespread in human populations, particularly in underdeveloped regions. Cereals such as rice (Oryza sativa) are the major dietary source of Zn for most people. However, the molecular mechanism underlying Zn uptake in rice is still not fully understood. Here, we report that a member of the ZIP (ZRT, IRT-like protein) family, OsZIP9, contributes to Zn uptake in rice. It was expressed in the epidermal and exodermal cells of lateral roots, localized in the plasma membrane and induced during Zn deficiency. Yeast-expressed OsZIP9 showed much higher Zn influx transport activity than other rice ZIP proteins in a wide range of Zn concentrations. OsZIP9 knockout rice plants showed a significant reduction in growth at low Zn concentrations, but could be rescued by a high Zn supply. Compared with the wild type, accumulation of Zn in root, shoot and grain was much lower in knockout lines, particularly with a low supply of Zn under both hydroponic and paddy soil conditions. OsZIP9 also showed Co uptake activity. Natural variation of OsZIP9 expression level is highly associated with Zn content in milled grain among rice varieties in the germplasm collection. Taken together, these results show that OsZIP9 is an important influx transporter responsible for the take up of Zn and Co from external media into root cells.

Details

ISSN :
1365313X and 09607412
Volume :
103
Database :
OpenAIRE
Journal :
The Plant Journal
Accession number :
edsair.doi.dedup.....74e4c0e9b422ee6b1c393e3f496cadd0
Full Text :
https://doi.org/10.1111/tpj.14855