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Experimental hepatobiliary injury. Comparison of in vivo drug elimination with in vitro drug-metabolizing enzyme capacity in the rat
- Source :
- Digestive diseases and sciences. 26(1)
- Publication Year :
- 1981
-
Abstract
- The effect of experimental hepatobiliary injury on drug metabolism was studied in a series ofin vivo andin vitro experiments in rats.In vivo aminopyrine elimination was measured by14CO2 breath analysis. This measure was compared with anin vitro estimate of the drug-metabolizing enzyme capacity of the whole liver (aminopyrine-free intrinsic clearance, Vmax/Km). The degree of hepatobiliary injury was measured by means of BSP biliary excretion. Two surgical models were studied: severe (complete bile duct obstruction) and mild (selective bile duct obstruction); as well as two drug-induced models: severe (alpha-naphthylisothiocyanate, ANIT) and mild (ethinyl estradiol). In the surgical models, only the complete-bile-duct-obstructed rat had a significantly decreased breath elimination rate of14CO2, 58% of control (P
- Subjects :
- medicine.medical_specialty
Physiology
Metabolic Clearance Rate
Hepatic Duct, Common
In Vitro Techniques
Ethinyl Estradiol
Cytochrome P-450 Enzyme System
In vivo
Internal medicine
medicine
Animals
Carbon Radioisotopes
Aminopyrine
Demethylation
chemistry.chemical_classification
Cholestasis
Bile duct
Chemistry
Gastroenterology
Metabolism
Hepatology
Carbon Dioxide
In vitro
Rats
medicine.anatomical_structure
Endocrinology
Enzyme
1-Naphthylisothiocyanate
Breath Tests
Liver
Drug metabolism
Aminopyrine N-Demethylase
Subjects
Details
- ISSN :
- 01632116
- Volume :
- 26
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Digestive diseases and sciences
- Accession number :
- edsair.doi.dedup.....74caf7b33ff0e179a3e942178f6c2444