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IL-1? and TNF? act synergistically to stimulate production of myeloid colony-stimulating factors by cultured human bone marrow stromal cells and cloned stromal cell strains
- Source :
- Journal of Cellular Physiology. 159:221-228
- Publication Year :
- 1994
- Publisher :
- Wiley, 1994.
-
Abstract
- Human bone marrow stromal cells respond to stimulation by the monokines IL-1 and TNF by producing colony-stimulating factors such as GM-CSF and G-CSF. In this study we show that IL-1 alpha and TNF alpha act synergistically to stimulate GM-CSF and G-CSF production by cultured marrow stromal cells. We further show that IL-1 alpha and TNF alpha synergistically stimulate production of GM-CSF and G-CSF by a clonal stroma-derived cell strain. Although IL-1 and TNF share many of the same biological activities, we show that IL-1 alpha and TNF alpha have an unequal ability to induce myeloid-CSF production by both cultures, with IL-1 alpha being the more potent inducer. We found that induction by IL-1 alpha and TNF alpha was independent of cell proliferation. The effect of IL-1 alpha and TNF alpha on production of the two myeloid-CSFs by the clonal cells was significantly greater than the unfractionated passaged stromal cultures, having the greater effect on G-CSF production. The clonally derived stromal cells constitutively produced colony-stimulating activity, in particular GM-CSF, at levels easily detected by ELISA. These findings show that, in addition to the overlapping and additive activities of IL-1 alpha and TNF alpha, they can interact synergistically. Our findings further suggest that a small subpopulation of stroma cells may be the major producer of G-CSF in the marrow microenvironment during immune response.
- Subjects :
- Myeloid
Stromal cell
Physiology
Clinical Biochemistry
Alpha (ethology)
Bone Marrow Cells
Biology
Bone Marrow
Granulocyte Colony-Stimulating Factor
medicine
Humans
Cells, Cultured
Tumor Necrosis Factor-alpha
Granulocyte-Macrophage Colony-Stimulating Factor
Drug Synergism
Cell Biology
Colony-stimulating factor
Recombinant Proteins
Clone Cells
Kinetics
Granulocyte macrophage colony-stimulating factor
medicine.anatomical_structure
Cell culture
Immunology
Cancer research
Tumor necrosis factor alpha
Bone marrow
Mitogens
Stromal Cells
Interleukin-1
medicine.drug
Subjects
Details
- ISSN :
- 10974652 and 00219541
- Volume :
- 159
- Database :
- OpenAIRE
- Journal :
- Journal of Cellular Physiology
- Accession number :
- edsair.doi.dedup.....74c1a8a9a6531b1bc3160e293c2519b6