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Translation of Virus mRNA: Synthesis of Bacteriophage Qβ Proteins in a Cell-Free Extract from Wheat Embryo
- Source :
- Journal of Virology. 12:1434-1441
- Publication Year :
- 1973
- Publisher :
- American Society for Microbiology, 1973.
-
Abstract
- The RNA from bacteriophage Qβ can be translated by cell-free extracts from wheat embryos. This translation, by 80 S ribosomes, occurs at a low magnesium ion concentration. Three products are synthesized which coelectrophorese with Qβ proteins synthesized in Escherichia coli extracts. The smallest of these has been identified as coat protein. Although the polycistronic bacteriophage message is translated with fidelity, the efficiency is much less than when the monocistronic brome mosaic virus coat protein message is translated.
- Subjects :
- Immunology
Tritium
medicine.disease_cause
Coliphages
Microbiology
Ribosome
Bacteriophage
Viral Proteins
Brome mosaic virus
Virology
Escherichia coli
medicine
Protein biosynthesis
Magnesium
Trypsin
Carbon Radioisotopes
RNA, Messenger
Amino Acids
Triticum
Cell-Free System
biology
Plant Extracts
food and beverages
RNA
biology.organism_classification
Biochemistry
Protein Biosynthesis
Insect Science
Bacterial Viruses
RNA, Viral
Electrophoresis, Polyacrylamide Gel
Peptides
Eukaryotic Ribosome
Ribosomes
Bacteriophage Qβ
Subjects
Details
- ISSN :
- 10985514 and 0022538X
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Journal of Virology
- Accession number :
- edsair.doi.dedup.....74b6754567c71bcfd9f78cdcc38ed9a6