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An animal model with a cardiomyocyte-specific deletion of estrogen receptor alpha: functional, metabolic, and differential network analysis
- Source :
- PLoS ONE, PLoS ONE, Vol 9, Iss 7, p e101900 (2014)
- Publication Year :
- 2014
-
Abstract
- Estrogen exerts diverse biological effects in multiple tissues in both animals and humans. Much of the accumulated knowledge on the role of estrogen receptor (ER) in the heart has been obtained from studies using ovariectomized mice, whole body ER gene knock-out animal models, ex vivo heart studies, or from isolated cardiac myocytes. In light of the wide systemic influence of ER signaling in regulating a host of biological functions in multiple tissues, it is difficult to infer the direct role of ER on the heart. Therefore, we developed a mouse model with a cardiomyocyte-specific deletion of the ERα allele (cs-ERα−/−). Male and female cs-ERα−/− mice with age/sex-matched wild type controls were examined for differences in cardiac structure and function by echocardiogram and differential gene expression microarray analysis. Our study revealed sex-differences in structural parameters in the hearts of cs-ERα−/− mice, with minimal functional differences. Analysis of microarray data revealed differential variations in the expression of 208 genes affecting multiple transcriptional networks. Furthermore, we report sex-specific differences in the expression of 56 genes. Overall, we developed a mouse model with cardiac-specific deletion of ERα to characterize the role of ERα in the heart independent of systemic effects. Our results suggest that ERα is involved in controlling the expression of diverse genes and networks in the cardiomyocyte in a sex-dependent manner.
- Subjects :
- Male
Microarrays
Estrogen receptor
lcsh:Medicine
Biochemistry
Mice
Cell Signaling
Gene expression
Medicine and Health Sciences
Gene Regulatory Networks
Myocytes, Cardiac
lcsh:Science
Oligonucleotide Array Sequence Analysis
Mice, Knockout
Multidisciplinary
Cell biology
Bioassays and Physiological Analysis
Cardiovascular Diseases
Female
Metabolic Pathways
Signal Transduction
Research Article
medicine.drug_class
Cardiology
Biology
Research and Analysis Methods
medicine
Sex Hormones
Cardiovascular Diseases in Women
Animals
Estrogen receptor beta
Microarray analysis techniques
Gene Expression Profiling
lcsh:R
Wild type
Estrogen Receptor alpha
Biology and Life Sciences
Estrogens
Cell Biology
Cardiovascular Disease Risk
Molecular biology
Hormones
Gene expression profiling
Mice, Inbred C57BL
Disease Models, Animal
Gene Ontology
Metabolism
Estrogen
lcsh:Q
Nuclear Receptor Signaling
Estrogen receptor alpha
Biomarkers
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 9
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- PloS one
- Accession number :
- edsair.doi.dedup.....74ae36910707df449cddf2059d4b89bb