Effect of pH on plating efficiency, serum reguirement, and incorporation of radioactive precursors into human cells

The plating efficiencies of two cell lines (HeLa-AT and WI26 Va) and one cell strain (KL2) appeared to be optimal at pH 7.6 At the optimal pH, ,sing organic buffers, the serum concentration could be reduced by one-half without affecting the initial growth rate or the final population density of the human skin fibroblast strain, KL2. It is suggested that an optimal pH lowers the serum requirement of the cells. The incorporation of [3-H]thymidine, [3-H]uridine, [-3H]fucose and [-14C]amino acids into KL2 was maximal at the pH which normally permits optimal growth of this cell strain. Protein turnover, as measured by loss to the medium of amino acids from prelabeled cells, was pH-independent. Thymidine incorporation did not correlate with culture growth in the cell strain, MS2A.