Additional file 6: of Reproducibility of Illumina platform deep sequencing errors allows accurate determination of DNA barcodes in cells

Impact of the offset of the log-likelihood threshold on the performance of the cleaning procedure. (A-D) The barcodes left after cleaning for variable log-likelihood offset thresholds are compared to the barcodes that are true according to the reference list of the viral barcode library. Considering the reference list as a gold standard, sensitivity, specificity and precision are shown for each of the four individual lanes. Dashed vertical lines denote the default value for the offset. Note that sensitivity declines with increasing offset, whereas specificity and precision increase, and the strongest effect is for large values of the offset. (PDF 32 kb)