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HER2 amplification and overexpression are significantly correlated in mucinous epithelial ovarian cancer
- Source :
- Human pathology. 45(4)
- Publication Year :
- 2013
-
Abstract
- HER2 gene amplification and protein over-expression are important factors in predicting clinical sensitivity to anti-HER2 therapies in breast, gastric or gastroesophageal junction cancer patients. The aim of this study was to evaluate the correlation between HER2 gene copy numbers and HER2 protein expressions in mucinous epithelial ovarian cancer (EOC). Of the 49 tissue microarray samples of mucinous EOC, we applied 2010 ToGA trial (Trastuzumab for Gastric Cancer) surgical specimen scoring criteria to analyze the HER2 protein expression by an immunohistochemistry (IHC) test with Dako (Carpenteria, CA), c-erb-B2 antibody, and the HER2 gene amplification by the fluorescence in situ hybridization (FISH) test with Abbott/Vysis PathVysion HER2 DNA Probe Kit (Abbott Molecular Inc., Des Plaines, IA). We achieved a high overall concordance of 97.56% between nonequivocal HER2 results by IHC and FISH tests. In addition, HER2 gene copies before chromosome-17 correction increased significantly in a stepwise order through the negative, equivocal and positive IHC result categories (P
- Subjects :
- Pathology
medicine.medical_specialty
Receptor, ErbB-2
Gene Dosage
Biology
Pathology and Forensic Medicine
Trastuzumab
medicine
Humans
skin and connective tissue diseases
neoplasms
Gene
In Situ Hybridization, Fluorescence
Ovarian Neoplasms
Tissue microarray
medicine.diagnostic_test
Hybridization probe
Gene Expression Profiling
Gene Amplification
Cancer
Genes, erbB-2
medicine.disease
Adenocarcinoma, Mucinous
Immunohistochemistry
Tissue Array Analysis
Cancer research
biology.protein
Female
Antibody
medicine.drug
Fluorescence in situ hybridization
Chromosomes, Human, Pair 17
Subjects
Details
- ISSN :
- 15328392
- Volume :
- 45
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Human pathology
- Accession number :
- edsair.doi.dedup.....748ebc2faa7827c144b6cda4cff55c57