Additional file 5: of Genomic, transcriptomic, and proteomic approaches towards understanding the molecular mechanisms of salt tolerance in Frankia strains isolated from Casuarina trees

Two-dimensional polyacrylamide gel electrophoresis (PAGE) analysis of Frankia sp. strain Allo2 under control (no stress) conditions (A), 200 mM NaCl (B), and 200 mM sucrose (C). Red arrows indicate that proteins are up-regulated relative to the control, while yellow arrows indicate down regulated proteins relative to the control. The corresponding number spots were in-gel digested with trypsin and analyzed by liquid chromatography-mass spectrometry (LC-MS) and LC-MS/MS for protein identification. (PPTX 653 kb)