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Extracellular superoxide dismutase in macrophages augments bacterial killing by promoting phagocytosis
- Source :
- The American journal of pathology. 178(6)
- Publication Year :
- 2010
-
Abstract
- Extracellular superoxide dismutase (EC-SOD) is abundant in the lung and limits inflammation and injury in response to many pulmonary insults. To test the hypothesis that EC-SOD has an important role in bacterial infections, wild-type and EC-SOD knockout (KO) mice were infected with Escherichia coli to induce pneumonia. Although mice in the EC-SOD KO group demonstrated greater pulmonary inflammation than did wild-type mice, there was less clearance of bacteria from their lungs after infection. Macrophages and neutrophils express EC-SOD; however, its function and subcellular localization in these inflammatory cells is unclear. In the present study, immunogold electron microscopy revealed EC-SOD in membrane-bound vesicles of phagocytes. These findings suggest that inflammatory cell EC-SOD may have a role in antibacterial defense. To test this hypothesis, phagocytes from wild-type and EC-SOD KO mice were evaluated. Although macrophages lacking EC-SOD produced more reactive oxygen species than did cells expressing EC-SOD after stimulation, they demonstrated significantly impaired phagocytosis and killing of bacteria. Overall, this suggests that EC-SOD facilitates clearance of bacteria and limits inflammation in response to infection by promoting bacterial phagocytosis.
- Subjects :
- Phagocytosis
Intracellular Space
Inflammation
Pathology and Forensic Medicine
Microbiology
Superoxide dismutase
Mice
medicine
Extracellular
Escherichia coli
Macrophage
Animals
Humans
Lung
chemistry.chemical_classification
Mice, Knockout
Reactive oxygen species
Microbial Viability
biology
Superoxide Dismutase
Macrophages
Regular Article
Pneumonia
Oxidants
Mice, Inbred C57BL
medicine.anatomical_structure
chemistry
biology.protein
medicine.symptom
Extracellular Space
Intracellular
Subjects
Details
- ISSN :
- 15252191
- Volume :
- 178
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- The American journal of pathology
- Accession number :
- edsair.doi.dedup.....74654e2161033d1cb11310d1d4e3fab6