Resveratrol-mediated SIRT1 activation attenuates ovalbumin-induced allergic rhinitis in mice

Resveratrol (RSV) is one kind of polyphenol that possesses anti-inflammatory effect. The effect of RSV in allergic rhinitis (AR) remains unclear. In this study, we investigated the protective effect of RSV against AR and explored the related signaling pathways. AR mouse model was successfully established by intraperitoneal injection with Ovalbumin (OVA). RSV administration was performed in a diet with different doses (5, 30, 50 mg/kg). Allergic symptoms were indicated by sneezing and nasal rubbing. Cytokines in serum and nasal lavage fluid (NLF) were determined by ELISA. mRNA and protein levels were detected by RT-PCR and Western blot. Our results show that RSV treatment attenuated sneezing and nasal rubbing in AR mice. It decreased histamine release, OVA-specific IgE, IgG1, IL-4 and LTC4, inflammatory cell numbers (leucocytes, eosinophils, lymphocytes, and neutrophils), and inflammatory cytokines secretion (TNF-α, IL-6, IL-10, IL-5, IL-13, and IL-17). Mechanically, RSV treatment suppressed HMGB1 and TLR4 expression and promoted SIRT1 expression in the nasal mucosa. In conclusion, RSV is able to attenuate OVA-induced AR in mice.