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Functional versus quantitative comparison of IL-1β from monocytes/macrophages on biomedical polymers
- Source :
- Journal of Biomedical Materials Research. 28:137-137
- Publication Year :
- 1994
- Publisher :
- Wiley, 1994.
-
Abstract
- Studies utilizing a quantitative assay, radioimmunoassay, and a biological activity assay, the murine thymocyte proliferation assay, to analyze IL-1 beta cytokine production by monocytes/macrophages on biomedical polymers have been carried out. Results indicate that the quantitative analysis of IL-1 beta on biomedical polymers and protein-adsorbed biomedical polymers is not indicative of and does not correlate with the results of the biological activity assay. IL-1 beta secreted from human monocytes/macrophages on Biomer, polydimethylsiloxane (PDMS), Dacron, polyethylene, expanded polytetrafluoroethylene, and control polystyrene with and without the preadsorption of physiological concentrations of human IgG, fibrinogen, and/or fibronectin was assayed. Quantitative levels of IL-1 beta suggest a greater functional response than that observed in the biological thymocyte proliferation assay when the polymers were studied directly or preadsorbed with IgG. On the other hand, preadsorption with fibrinogen or fibronectin resulted in high functional activity for IL-1 beta with low quantitative levels of IL-1 beta. The lack of correlation between the functional and biological activity assays suggests the presence of other cytokines or antagonists which modulate the biological activity of IL-1 beta.
- Subjects :
- medicine.medical_treatment
Biomedical Engineering
Biocompatible Materials
Fibrinogen
Monocytes
Biomaterials
Isoantibodies
medicine
Humans
Monocytes macrophages
Beta (finance)
Cells, Cultured
biology
Cell-Free System
Chemistry
Monocyte
Macrophages
Biomedical polymers
Biological activity
Radioimmunoassay
Molecular biology
Fibronectin
Cytokine
medicine.anatomical_structure
Biochemistry
Immunology
biology.protein
Quantitative analysis (chemistry)
medicine.drug
Interleukin-1
Subjects
Details
- ISSN :
- 10974636 and 00219304
- Volume :
- 28
- Database :
- OpenAIRE
- Journal :
- Journal of Biomedical Materials Research
- Accession number :
- edsair.doi.dedup.....7455bd8099f320d90d1b283fd55a40b8