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Ankrd31 nécessaire à l'intégrité du sperme et de l'épididyme
- Source :
- Frontiers in Cell and Developmental Biology, Frontiers in Cell and Developmental Biology, Frontiers media, 2021, 9, pp.1-17. ⟨10.3389/fcell.2021.741975⟩, Frontiers in Cell and Developmental Biology, Frontiers media, 2021, 9, ⟨10.3389/fcell.2021.741975⟩, Frontiers in Cell and Developmental Biology, 2021, 9, 17 p. ⟨10.3389/fcell.2021.741975⟩, Frontiers in Cell and Developmental Biology, Vol 9 (2021)
- Publication Year :
- 2021
-
Abstract
- Ankyrin proteins (ANKRD) are key mediators linking membrane and sub-membranous cytoskeletal proteins. Recent findings have highlighted a new role of ANKRD31 during spermatogenesis, elucidating its involvement in meiotic recombination and male germ cell progression. Following testicular differentiation, spermatozoa (SPZ) enter into the epididymis, where they undergo several biochemical and enzymatic changes. The epididymal epithelium is characterized by cell-to-cell junctions that are able to form the blood-epididymal barrier (BEB). This intricate epithelial structure provides the optimal microenvironment needed for epididymal sperm maturation. To date, no notions have been reported regarding a putative role of ANKRD31 in correct BEB formation. In our work, we generated an Ankrd31 knockout male mouse model (Ankrd31–/–) and characterized its reproductive phenotype. Ankrd31–/– mice were infertile and exhibited oligo-astheno-teratozoospermia (a low number of immotile SPZ with abnormal morphological features). In addition, a complete deregulation of BEB was found in Ankrd31–/–, due to cell-to-cell junction anomalies. In order to suggest that BEB deregulation may depend on Ankrd31 gene deletion, we showed the physical interaction among ANKRD31 and some epithelial junction proteins in wild-type (WT) epididymides. In conclusion, the current work shows a key role of ANKRD31 in the control of germ cell progression as well as sperm and epididymal integrity.
- Subjects :
- sperm quality
QH301-705.5
ankyrins
[SDV]Life Sciences [q-bio]
Biology
male infertility
Male infertility
[SDV.BDLR.RS]Life Sciences [q-bio]/Reproductive Biology/Sexual reproduction
03 medical and health sciences
Cell and Developmental Biology
0302 clinical medicine
blood-epididymal-barrier
medicine
Ankyrin
Biology (General)
030304 developmental biology
Original Research
chemistry.chemical_classification
0303 health sciences
030219 obstetrics & reproductive medicine
Cell Biology
medicine.disease
Epididymis
Sperm
Phenotype
spermatogenesis
Cell biology
[SDV] Life Sciences [q-bio]
medicine.anatomical_structure
chemistry
Homologous recombination
Spermatogenesis
Germ cell
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 2296634X
- Database :
- OpenAIRE
- Journal :
- Frontiers in Cell and Developmental Biology, Frontiers in Cell and Developmental Biology, Frontiers media, 2021, 9, pp.1-17. ⟨10.3389/fcell.2021.741975⟩, Frontiers in Cell and Developmental Biology, Frontiers media, 2021, 9, ⟨10.3389/fcell.2021.741975⟩, Frontiers in Cell and Developmental Biology, 2021, 9, 17 p. ⟨10.3389/fcell.2021.741975⟩, Frontiers in Cell and Developmental Biology, Vol 9 (2021)
- Accession number :
- edsair.doi.dedup.....744edcfc2fe183bdfc12ee65224007e1