Supplementary Figure A from Accelerated Tumor Growth Mediated by Sublytic Levels of Antibody-Induced Complement Activation Is Associated with Activation of the PI3K/AKT Survival Pathway

Supplementary Figure B - PDF file 42K, CHLA136Luc or H524 cells treated with Human sera Complement (HuC') or heat inactivated HuC'(De-HuC') with or without the mAbs (3F8, PGNX and R24) for overnight. De- HuC' was achieved by heating the human complement for 30 min at 56{degree sign}C. Plates were read 2-4hrs after WST-1 addition (1:10 ratio) at 415nm. Figures representing the percent change in cell growth calculated according to formula: (OD of mAb+HuC' or de-HuC' - OD of HuC' or de-HuC')/OD of HuC' or de-HuC') X 100