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Cdc14 Inhibition by the Spindle Assembly Checkpoint Prevents Unscheduled Centrosome Separation in Budding Yeast
- Source :
- Molecular Biology of the Cell. 20:2626-2637
- Publication Year :
- 2009
- Publisher :
- American Society for Cell Biology (ASCB), 2009.
-
Abstract
- The spindle assembly checkpoint (SAC) is an evolutionarily conserved surveillance mechanism that delays anaphase onset and mitotic exit in response to the lack of kinetochore attachment. The target of the SAC is the E3 ubiquitin ligase anaphase-promoting complex (APC) bound to its Cdc20 activator. The Cdc20/APC complex is in turn required for sister chromatid separation and mitotic exit through ubiquitin-mediated proteolysis of securin, thus relieving inhibition of separase that unties sister chromatids. Separase is also involved in the Cdc-fourteen early anaphase release (FEAR) pathway of nucleolar release and activation of the Cdc14 phosphatase, which regulates several microtubule-linked processes at the metaphase/anaphase transition and also drives mitotic exit. Here, we report that the SAC prevents separation of microtubule-organizing centers (spindle pole bodies [SPBs]) when spindle assembly is defective. Under these circumstances, failure of SAC activation causes unscheduled SPB separation, which requires Cdc20/APC, the FEAR pathway, cytoplasmic dynein, and the actin cytoskeleton. We propose that, besides inhibiting sister chromatid separation, the SAC preserves the accurate transmission of chromosomes also by preventing SPBs to migrate far apart until the conditions to assemble a bipolar spindle are satisfied.
- Subjects :
- Saccharomyces cerevisiae Proteins
Cell Cycle Proteins
BIO/18 - GENETICA
Spindle Apparatus
CDC20
Protein Serine-Threonine Kinases
Biology
Microtubules
Anaphase-Promoting Complex-Cyclosome
Endopeptidases
Molecular Biology
Alleles
Separase
Anaphase
Centrosome
Kinetochore
Dyneins
Ubiquitin-Protein Ligase Complexes
Articles
Cell Biology
Actins
Spindle apparatus
Cell biology
spindle assembly checkpoint, Cdc14, spindle pole body, sister chromatid separation, genome integrity
Spindle checkpoint
Mitotic exit
Mutation
Saccharomycetales
Protein Tyrosine Phosphatases
Anaphase-promoting complex
Subjects
Details
- ISSN :
- 19394586 and 10591524
- Volume :
- 20
- Database :
- OpenAIRE
- Journal :
- Molecular Biology of the Cell
- Accession number :
- edsair.doi.dedup.....7442abbec8d2e13670e29c44b28455c1
- Full Text :
- https://doi.org/10.1091/mbc.e08-11-1150