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Gene amplification in non-Hodgkin's lymphoma
- Source :
- British Journal of Haematology. 86:792-797
- Publication Year :
- 1994
- Publisher :
- Wiley, 1994.
-
Abstract
- Among 426 consecutively ascertained and karyotypically abnormal non-Hodgkin's lymphoma (NHL) tumours, cytological evidence for gene amplification in the form of homogeneously staining regions (HSRs) was encountered in nine cases of large cell diffuse lymphoma (LC-DL). The mean age of patients with HSRs was 62.9 years and four died within a year of diagnosis. To identify candidate gene(s) amplified in these tumours, we performed a Southern blot analysis of tumour DNA using probes for 23 known protooncogenes and the multidrug resistance gene, PGY1. Besides a two-fold amplification of the BCL2 gene in two cases, no evidence for overt amplification of any of the genes assayed was found. To confirm DNA amplification in these specimens we performed the DNA in-gel renaturation assay. Evidence for presence of amplified DNA fragments was obtained in four of seven specimens. These results suggest amplification of a novel gene(s). To our knowledge, this is the first formal study of gene amplification in a large consecutively ascertained series of fresh lymphoma biopsies.
- Subjects :
- Adult
Male
medicine.medical_specialty
Candidate gene
Biology
Proto-Oncogenes
Gene duplication
medicine
Humans
Double minute
Gene
Aged
Southern blot
Chromosome Aberrations
Lymphoma, Non-Hodgkin
Gene Amplification
Cytogenetics
DNA, Neoplasm
Hematology
Middle Aged
medicine.disease
Molecular biology
Lymphoma
Non-Hodgkin's lymphoma
Blotting, Southern
Karyotyping
Nucleic Acid Renaturation
Female
Subjects
Details
- ISSN :
- 13652141 and 00071048
- Volume :
- 86
- Database :
- OpenAIRE
- Journal :
- British Journal of Haematology
- Accession number :
- edsair.doi.dedup.....743a381d12833e2f23362055036891db