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Genome organization of membrane-containing bacteriophage PRD1
- Source :
- Virology. 183(2)
- Publication Year :
- 1991
-
Abstract
- We have determined the nucleotide sequence of the late region (11 kbp) of the lipid-containing bacteriophage PRD1. Gene localization was carried out by complementing nonsense phage mutants with genomic clones containing specific reading frames. The localization was confirmed by sequencing the N-termini of isolated gene products as well as sequencing the N-termini of tryptic fragments of the phage membrane-associated proteins. This, with the previously obtained sequence of the early regions, allowed us to organize most of the phage genes in the phage genome.
- Subjects :
- Phage display
Genes, Viral
viruses
Phagemid
Molecular Sequence Data
Restriction Mapping
Biology
Genome
Bacteriophage
03 medical and health sciences
Open Reading Frames
Viral Envelope Proteins
Virology
Gram-Negative Bacteria
Bacteriophages
Amino Acid Sequence
Cloning, Molecular
Gene
030304 developmental biology
Genomic organization
Genetics
0303 health sciences
Base Sequence
030306 microbiology
Genetic Complementation Test
Nucleic acid sequence
Tectivirus
biology.organism_classification
DNA, Viral
Mutation
Electrophoresis, Polyacrylamide Gel
Subjects
Details
- ISSN :
- 00426822
- Volume :
- 183
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Virology
- Accession number :
- edsair.doi.dedup.....7437c0e844f529a753598abf3077435c