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Comparative analysis of immune repertoires between Bactrian camel's conventional and heavy-chain antibodies
- Source :
- Li, X, Duan, X, Yang, K, Zhang, W, Zhang, C, Fu, L, Ren, Z, Wang, C, Wu, J, Lu, R, Ye, Y, He, M, Nie, C, Yang, N, Wang, J, Yang, H, Liu, X & Tan, W 2016, ' Comparative analysis of immune repertoires between Bactrian camel's conventional and heavy-chain antibodies ', P L o S One, vol. 11, no. 9, e0161801 . https://doi.org/10.1371/journal.pone.0161801, PLoS ONE, Vol 11, Iss 9, p e0161801 (2016), PLoS ONE
- Publication Year :
- 2016
-
Abstract
- Compared to classical antibodies, camel heavy chain antibodies (HCAbs) are smaller in size due to lack of the light chain and the first constant domain of the heavy chain (CH1 region). The variable regions of HCAbs (VHHs) are more soluble and stable than that of conventional antibodies (VHs). Even with such simple structure, they are still functional in antigen binding. Although HCAbs have been extensively investigated over the past two decades, most efforts have been based upon low throughput sequence analysis, and there are only limited reports trying to analyze and describe the complete immune repertoire (IR) of camel HCAbs. Here we leveraged the high-throughput data generated by Next Generation Sequencing (NGS) of the variable domains of the antibody heavy chains from three Bactrian camels to conduct in-depth comparative analyses of the immunoglobulin repertoire. These include analyses of the complementary determining region 3 (CDR3) length and distribution, mutation rate, antibody characteristic amino acids, the distribution of the cysteine (Cys) codons, and the non-classical VHHs. We found that there is higher diversity in the CDR2 than in the other sub-regions, and there is a higher mutation rate in the VHHs than in the VHs (P < 0.05). In addition to substitutions at amino acid (AA) residue positions NO.49/50/52 between VH and VHH clones, we also observed other substitutions at the positions NO.40/54/57/96/101 that could lead to additional structural alterations. We also found that VH-derived VHH clones, referred to as non-classical VHH clones in this study, accounted for about 8% of all clones. Further, only 5%-10% clones had the Trp > Arg AA substitution at the first position of framework 4 for all types of clones. We present, for the first time, a relatively complete picture of the Bactrian camel antibody immune repertoire, including conventional antibody (Ab) and HCAbs, using PCR and in silico analysis based on high-throughput NGS data.
- Subjects :
- 0301 basic medicine
Mutation rate
Physiology
Immunoglobulin Variable Region
lcsh:Medicine
Artificial Gene Amplification and Extension
Biochemistry
Polymerase Chain Reaction
0302 clinical medicine
Camels
Immune Physiology
Medicine and Health Sciences
lcsh:Science
Mammals
Multidisciplinary
Immune System Proteins
Genes, Immunoglobulin
High-Throughput Nucleotide Sequencing
Vertebrates
Amino Acid Analysis
Antibody
Immunoglobulin Heavy Chains
Sequence Analysis
Research Article
Camelus
Sequence analysis
In silico
Immunology
Nucleotide Sequencing
Sequence alignment
Computational biology
Biology
Molecular cloning
Immunoglobulin light chain
Research and Analysis Methods
Antibodies
03 medical and health sciences
Animals
Bactrian camel
Molecular Biology Techniques
Sequencing Techniques
Molecular Biology
Molecular Biology Assays and Analysis Techniques
lcsh:R
Organisms
Biology and Life Sciences
Proteins
biology.organism_classification
Molecular biology
030104 developmental biology
Amniotes
biology.protein
lcsh:Q
Sequence Alignment
030215 immunology
Cloning
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Li, X, Duan, X, Yang, K, Zhang, W, Zhang, C, Fu, L, Ren, Z, Wang, C, Wu, J, Lu, R, Ye, Y, He, M, Nie, C, Yang, N, Wang, J, Yang, H, Liu, X & Tan, W 2016, ' Comparative analysis of immune repertoires between Bactrian camel's conventional and heavy-chain antibodies ', P L o S One, vol. 11, no. 9, e0161801 . https://doi.org/10.1371/journal.pone.0161801, PLoS ONE, Vol 11, Iss 9, p e0161801 (2016), PLoS ONE
- Accession number :
- edsair.doi.dedup.....74301e5342dd15f0c291614324bbfc7c