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Notch 1 Signaling Regulates Peripheral T Cell Activation
- Source :
- Immunity. 20:407-415
- Publication Year :
- 2004
- Publisher :
- Elsevier BV, 2004.
-
Abstract
- Notch signaling has been identified as an important regulator of leukocyte differentiation and thymic maturation. Less is known about the role of Notch signaling in regulating mature T cells. We examined the role of Notch 1 in regulating peripheral T cell activity in vitro and in vivo. Coligation of Notch 1 together with TCR and CD28 resulted in a dramatic inhibition of T cell activation, proliferation, and cytokine production. This effect was dependent on presenilin activity and induced the expression of HES-1, suggestive of Notch 1 signaling. Biochemical analysis demonstrated an inhibition of AKT and GSK3β phosphorylation following Notch 1 engagement while other biochemical signals such as TCR and ERK phosphorylation remained intact. Similar effects were observed in vivo in an adoptive transfer model. Therefore, Notch 1 signaling may play an important role in regulating naive T cell activation and homeostasis.
- Subjects :
- Naive T cell
T-Lymphocytes
T cell
Blotting, Western
Immunology
Receptors, Antigen, T-Cell
Notch signaling pathway
Receptors, Cell Surface
Biology
Lymphocyte Activation
Transfection
Cell Line
Mice
03 medical and health sciences
0302 clinical medicine
CD28 Antigens
medicine
Animals
Humans
Immunology and Allergy
Receptor, Notch1
Notch 1
030304 developmental biology
0303 health sciences
T-cell receptor
CD28
Flow Cytometry
Adoptive Transfer
Precipitin Tests
Cell biology
Infectious Diseases
medicine.anatomical_structure
Hes3 signaling axis
Cyclin-dependent kinase 8
Cell Division
Signal Transduction
Transcription Factors
030215 immunology
Subjects
Details
- ISSN :
- 10747613
- Volume :
- 20
- Database :
- OpenAIRE
- Journal :
- Immunity
- Accession number :
- edsair.doi.dedup.....742da9a24fbe2bb8571cbcdc77518168