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The mechanism research of non-Smad dependent TAK1 signaling pathway in the treatment of bone defects by recombination BMP-2-loaded hollow hydroxyapatite microspheres/chitosan composite
- Source :
- Journal of Materials Science: Materials in Medicine. 30
- Publication Year :
- 2019
- Publisher :
- Springer Science and Business Media LLC, 2019.
-
Abstract
- The present study aimed to evaluate whether the non-Smad dependent TAK1 signaling pathway (BMP-2-TAK1-p38-Osx signaling pathway) played an important role in bone repair mediated by hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite. Firstly, the biological activity of rhBMP-2 released from the complex was investigated. Then, differentiation test of osteoblasts including ALP activity and calcium deposition, X-ray scoring and three-point bending test were performed. Finally, the mRNAs expression of TAK1, p38, Osx and osteogenic markers was tested by reverse transcription-polymerase chain reaction (RT-PCR). RhBMP-2 could be loaded and released from the complex in bioactive form. Additionally, the complex provided a prolonged period of time compared with HA/CS scaffolds. Serum ALP activity was significantly decreased in the TAK1 inhibitor group and p38 inhibitor group. In the X-ray radiography, bone callus was observed in rhBMP-2-loaded hollow HA microspheres/CS composite group. In the three-point bending test, load values in p38 inhibitor group decreased. In the animal model, the mRNA expression of BSP on day 90 was significantly decreased in the p38 inhibitor group and TAK1 inhibitor group. In MC3T3-E1 cells, the mRNA expression of OSX was remarkably up-regulated in both rhBMP-2 group or rhBMP-2-loaded hollow HA microspheres/CS composite group; while the mRNA expression of OSX was significantly down-regulated in TAK1 inhibitor group and p38 inhibitor group. The BMP-2-TAK1-p38-OSX signaling pathway may play an important role in bone formation and repair mediated by rhBMP-2-loaded hollow HA microspheres/CS composite.
- Subjects :
- Male
Bone Regeneration
Materials science
p38 mitogen-activated protein kinases
0206 medical engineering
Biomedical Engineering
Biophysics
Bone Morphogenetic Protein 2
Biocompatible Materials
Bioengineering
02 engineering and technology
SMAD
Bone healing
Bone morphogenetic protein 2
Bone and Bones
Biomaterials
Chitosan
chemistry.chemical_compound
Osteogenesis
Transforming Growth Factor beta
Materials Testing
Animals
Bone regeneration
Biological activity
021001 nanoscience & nanotechnology
020601 biomedical engineering
Microspheres
Recombinant Proteins
Cell biology
Durapatite
chemistry
Bone Substitutes
Rabbits
Signal transduction
0210 nano-technology
Subjects
Details
- ISSN :
- 15734838 and 09574530
- Volume :
- 30
- Database :
- OpenAIRE
- Journal :
- Journal of Materials Science: Materials in Medicine
- Accession number :
- edsair.doi.dedup.....741b45887e9dfa8119689124da85cd03