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Photobiomodulation (660 nm) therapy reduces oxidative stress and induces BDNF expression in the hippocampus
- Source :
- Scientific Reports, Vol 9, Iss 1, Pp 1-8 (2019), Scientific Reports
- Publication Year :
- 2019
- Publisher :
- Nature Publishing Group, 2019.
-
Abstract
- Photobiomodulation therapy (PBMT) effects an important role in neural regeneration and function enhancement, such as expression of nerve growth factor and nerve regeneration, in neuronal tissues, and inhibition of cell death by amyloid beta in neurons is inhibited by PBMT. However, there no studies evaluated the effects of PBMT on oxidative stress in the hippocampus. The aim of this study is to evaluate the effects of PBMT on oxidative stress in the hippocampus. This study assessed the anti-oxidative effect, the expression of BDNF and antioxidant enzymes, as well as the activation of cAMP response element binding (CREB) and extracellular signal-regulated kinase (ERK) signal transduction pathways assess using a hippocampal cell line (HT-22) and mouse organotypic hippocampal tissues by PBMT (LED, 660 nm, 20 mW/cm2). PBMT inhibited HT-22 cell death by oxidative stress and increased BDNF expression via ERK and CREB signaling pathway activation. In addition, PBMT increased BDNF expression in hippocampal organotypic slices and the levels of phosphorylated ERK and CREB, which were reduced by oxidative stress, as well as the expression of the antioxidant enzyme superoxide dismutase. These data demonstrate that PBMT inhibits hippocampal damage induced by oxidative stress and increases the expression of BDNF, which can be used as an alternative to treat a variety of related disorders that lead to nerve damage. Activation and redox homeostasis in neuronal cells may be a notable mechanism of the 660-nm PBMT-mediated photobioreactivity.
- Subjects :
- 0301 basic medicine
MAPK/ERK pathway
MAP Kinase Signaling System
Cell death in the nervous system
lcsh:Medicine
Hippocampal formation
CREB
medicine.disease_cause
Hippocampus
Article
Antioxidants
Superoxide dismutase
03 medical and health sciences
0302 clinical medicine
Organ Culture Techniques
medicine
Animals
Low-Level Light Therapy
Cyclic AMP Response Element-Binding Protein
lcsh:Science
CAMP response element binding
Multidisciplinary
biology
Cell Death
Chemistry
Brain-Derived Neurotrophic Factor
lcsh:R
Cognitive neuroscience
Cell biology
Enzymes
Mice, Inbred C57BL
Oxidative Stress
030104 developmental biology
Nerve growth factor
biology.protein
lcsh:Q
Signal transduction
030217 neurology & neurosurgery
Oxidative stress
Signal Transduction
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 9
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....7410a3174f31738c18cf45da4983e7d9
- Full Text :
- https://doi.org/10.1038/s41598-019-46490-4