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Blind unmixing of spectrally resolved lifetime images
- Source :
- Journal of Biomedical Optics. 18:086006
- Publication Year :
- 2013
- Publisher :
- SPIE-Intl Soc Optical Eng, 2013.
-
Abstract
- A method, is presented for blind unmixing spectrally resolved fluorescence lifetime images. The method is based on the combined analysis of spectral and lifetime phasors and allows unmixing of up to three components without any prior knowledge. Fractional intensities, spectra and decay curves of the individual components can be extracted with this new technique. The reliability and sensitivity are investigated and the possibility of extending the method to unmix more components is discussed. The method is evaluated on mixtures of fluorescent dyes and labeled cells.
- Subjects :
- Photon
Materials science
Biomedical Engineering
Sensitivity and Specificity
Spectral line
Pattern Recognition, Automated
Biomaterials
symbols.namesake
Optics
Image Interpretation, Computer-Assisted
Microscopy
Sensitivity (control systems)
business.industry
Reproducibility of Results
Image segmentation
Image Enhancement
Fluorescence
Atomic and Molecular Physics, and Optics
Molecular Imaging
Electronic, Optical and Magnetic Materials
Spectrometry, Fluorescence
Fourier transform
Microscopy, Fluorescence
Subtraction Technique
symbols
RGB color model
business
Algorithms
Subjects
Details
- ISSN :
- 10833668
- Volume :
- 18
- Database :
- OpenAIRE
- Journal :
- Journal of Biomedical Optics
- Accession number :
- edsair.doi.dedup.....7407d91471b939f9e689033d99aa4171
- Full Text :
- https://doi.org/10.1117/1.jbo.18.8.086006