Supplementary Methods, Figures 1 - 7 from Base Excision Repair Defects Invoke Hypersensitivity to PARP Inhibition

PDF file - 422KB, S1. Cell survival as measured by a clonogenic assay. S2. BRCT I domain of XRCC1. S3. Characterization of cell lines by western blotting. S4. Characterization of full-length wild-type XRCC1 and L360R mutant XRCC1 mouse proteins. S5. Inhibitory effect of olaparib (O) or veliparib (V) (10 mM) on PAR accumulation in MMS-treated (10 mM for 20 min at 4 degrees C) wild-type (red) and pol beta null cells (gray). S6. Effect of PARPi on sensitivity of wild-type (16.3, closed red symbols) and pol beta null (19.4, open black symbols) cells to MMS. S7. Sensitivity of pol beta- and XRCC1-deficient cell lines to olaparib and veliparib.