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Hypoxic cell turnover in different solid tumor lines
- Source :
- International Journal of Radiation Oncology, Biology, Physics, 62, 1157-68, International Journal of Radiation Oncology, Biology, Physics, 62, 4, pp. 1157-68
- Publication Year :
- 2004
-
Abstract
- Contains fulltext : 47332.pdf (Publisher’s version ) (Closed access) PURPOSE: Most solid tumors contain hypoxic cells, and the amount of tumor hypoxia has been shown to have a negative impact on the outcome of radiotherapy. The efficacy of combined modality treatments depends both on the sequence and timing of the treatments. Hypoxic cell turnover in tumors may be important for optimal scheduling of combined modality treatments, especially when hypoxic cell targeting is involved. METHODS AND MATERIALS: Previously we have shown that a double bioreductive hypoxic marker assay could be used to detect changes of tumor hypoxia in relation to the tumor vasculature after carbogen and hydralazine treatments. This assay was used in the current study to establish the turnover rate of hypoxic cells in three different tumor models. The first hypoxic marker, pimonidazole, was administered at variable times before tumor harvest, and the second hypoxic marker, CCI-103F, was injected at a fixed time before harvest. Hypoxic cell turnover was defined as loss of pimonidazole (first marker) relative to CCI-103F (second marker). RESULTS: The half-life of hypoxic cell turnover was 17 h in the murine C38 colon carcinoma line, 23 h and 49 h in the human xenograft lines MEC82 and SCCNij3, respectively. Within 24 h, loss of pimonidazole-stained areas in C38 and MEC82 occurred concurrent with the appearance of pimonidazole positive cell debris in necrotic regions. In C38 and MEC82, most of the hypoxic cells had disappeared after 48 h, whereas in SCCNij3, viable cells that had been labeled with pimonidazole were still observed after 5 days. CONCLUSIONS: The present study demonstrates that the double hypoxia marker assay can be used to study changes in both the proportion of hypoxic tumor cells and their lifespan at the same time. The present study shows that large differences in hypoxic cell turnover rates may exist among tumor lines, with half-lives ranging from 17-49 h.
- Subjects :
- Cancer Research
Pathology
medicine.medical_specialty
Time Factors
Cell Survival
medicine.medical_treatment
Mice, Nude
Mice
Carbogen
Translational research [ONCOL 3]
Interventional oncology [UMCN 1.5]
Cell Line, Tumor
Neoplasms
medicine
Pimonidazole
Animals
Humans
Radiology, Nuclear Medicine and imaging
Large intestine
Coloring Agents
Mice, Inbred BALB C
Radiation
Tumor hypoxia
business.industry
Hydralazine
Hypoxia (medical)
Cell Hypoxia
Radiation therapy
medicine.anatomical_structure
Oncology
Cell culture
Nitroimidazoles
Cancer research
Benzimidazoles
medicine.symptom
business
medicine.drug
Half-Life
Subjects
Details
- ISSN :
- 03603016
- Volume :
- 62
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- International journal of radiation oncology, biology, physics
- Accession number :
- edsair.doi.dedup.....73f9eb0441cbbc9034ca2eb46edc0a5b