LIM-Homeodomain Gene Family in Neural Development

In the present study, we isolated five murine LIM-homeodomain (LH) genes including a novel gene designated as L3 which is expressed specifically in the medial ganglionic eminence of the embryonic brain and the mesenchyme surrounding the oral cavity. The comparison of the expression domains in the embryonic forebrain using in situ hybridization histochemistry proved that three LH genes (LH-2, lim-1, and L3) share sharp boundaries. In addition to the prosomeric boundaries in the diencephalon, this gene family defines novel boundaries in the basal telencephalon, the mesencephalon, and the rhombencephalon. These mutually exclusive expression domains suggest that this family might be involved in controlling the early compartmentalization and boundary formation of the developing central nervous system.