Back to Search
Start Over
Activation of cord T lymphocytes. I. Evidence for a defective T cell mitogenesis induced through the CD2 molecule
- Source :
- The Journal of Immunology. 142:2583-2589
- Publication Year :
- 1989
- Publisher :
- The American Association of Immunologists, 1989.
-
Abstract
- A study was carried out on cord blood T cell activation via the CD2-mediated pathway. Despite similar percentages of circulating CD3+ and CD2+ cells in adult and cord blood, the proliferation of cord PBMC to the anti-CD3 mAb and cord T cells to anti-CD2 mAb were defective. The T cell CD3-surface structure was normally able to control CD2-mediated activation, as its modulation by a non-mitogenic anti-CD3 mAb blocked cord PBMC proliferation induced by anti-CD2 mAb. CD2-stimulated cord T cells did not proliferate and did not produce a significant amount of IL-2 in culture, although they expressed the IL-2R. This observation was confirmed by the optimal proliferation of CD2-induced cord T cells when rIL-2 was added. Despite the alternative T cell activation pathway is monocyte-independent in adults, the defective cord T cell activation via the CD2 molecule could also be bypassed by the addition of PMA, small amounts of either autologous or allogeneic adult and cord AC or simply rIL-1 alone. Our findings provide evidence for an intrinsic functional defect in cord CD2-mediated T cell activation, which is linked to an impaired increase of free cytoplasmic calcium, as confirmed by the effectiveness of calcium ionophore A23187 in restoring a good CD2-induced cord T cell proliferation and by measurement of cellular calcium uptake after activation via the CD2 molecule. The characteristics of cord T cells revealed by this study recall the thymocyte functional pattern and may represent functional expression of the previously described phenotypic immaturity of cord T cells.
- Subjects :
- Antigens, Differentiation, T-Lymphocyte
CD3 Complex
T-Lymphocytes
Immunology
CD2 Antigens
Receptors, Antigen, T-Cell
Antibodies, Monoclonal
Antigen-Presenting Cells
Receptors, Interleukin-2
Fetal Blood
Lymphocyte Activation
Humans
Interleukin-2
Immunology and Allergy
Calcium
Phytohemagglutinins
Receptors, Immunologic
Interleukin-1
Subjects
Details
- ISSN :
- 15506606 and 00221767
- Volume :
- 142
- Database :
- OpenAIRE
- Journal :
- The Journal of Immunology
- Accession number :
- edsair.doi.dedup.....73e9dde8dbc216caea150079e067c75b