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Enhancement of facultative anaerobic denitrifying communities by oxygen release from roots of the macrophyte in constructed wetlands
- Source :
- Journal of Environmental Management. 246:157-163
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- The radial oxygen loss (ROL) of wetland plants is a crucial factor that can influence the efficiency required for nitrogen (N) removal and microbial activities responsible for N removal in constructed wetlands (CWs). However, the shift of microbial community in different niches in response to ROL has been rarely studied. This study aims to unravel the link between the ROL and microbial response in sediment, water and rhizoplane by a surface flow CW planted with Myriophyllum aquaticum for treating high-strength swine wastewater. Ti3+-citrate colorimetric method demonstrated that M. aquaticum was a wetland species with a ROL of 0.019 mg/h/plant. Using quantitative polymerase chain reactions (qPCR) and high-throughput sequencing of microbial 16S rRNA gene, we demonstrated that the abundance of facultative anaerobic denitrifiers in the rhizoplane was the most of the three niches, that in the water (5–10 cm) was the less and that in the sediment was the least. Acinetobacter was enriched and dominated amongst denitrifiers in the water. Denitrifiers in the rhizoplane were mainly dominated by enriched Pseudomonas, Aeromonas, and Acinetobacter. The theoretical calculation of oxygen sources and consumptions indicated that water reaeration should support the oxygen demands for nitrification in the aerobic layer (0–5 cm), and the ROL could stimulate the growth of facultative anaerobic denitrifiers in the rhizoplane and water (5–10 cm) to achieve denitrification within CW systems.
- Subjects :
- Environmental Engineering
Denitrification
Nitrogen
Swine
0208 environmental biotechnology
Wetland
02 engineering and technology
Wastewater
010501 environmental sciences
Management, Monitoring, Policy and Law
01 natural sciences
Denitrifying bacteria
RNA, Ribosomal, 16S
Animals
Anaerobiosis
Waste Management and Disposal
0105 earth and related environmental sciences
Facultative
geography
geography.geographical_feature_category
biology
Chemistry
Myriophyllum aquaticum
food and beverages
General Medicine
biology.organism_classification
020801 environmental engineering
Macrophyte
Oxygen
Microbial population biology
Wetlands
Environmental chemistry
Nitrification
Subjects
Details
- ISSN :
- 03014797
- Volume :
- 246
- Database :
- OpenAIRE
- Journal :
- Journal of Environmental Management
- Accession number :
- edsair.doi.dedup.....73e2e8e716142a914b5b120a724fe233