Back to Search
Start Over
Multiplexed, quantitative serological profiling of COVID-19 from blood by a point-of-care test
- Source :
- medRxiv, article-version (status) pre, article-version (number) 1, Science Advances
- Publication Year :
- 2021
-
Abstract
- A point-of-care, microfluidic platform detects antibodies against three SARS-CoV-2 antigens from blood in less than an hour.<br />Highly sensitive, specific, and point-of-care (POC) serological assays are an essential tool to manage coronavirus disease 2019 (COVID-19). Here, we report on a microfluidic POC test that can profile the antibody response against multiple severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antigens—spike S1 (S1), nucleocapsid (N), and the receptor binding domain (RBD)—simultaneously from 60 μl of blood, plasma, or serum. We assessed the levels of antibodies in plasma samples from 31 individuals (with longitudinal sampling) with severe COVID-19, 41 healthy individuals, and 18 individuals with seasonal coronavirus infections. This POC assay achieved high sensitivity and specificity, tracked seroconversion, and showed good concordance with a live virus microneutralization assay. We can also detect a prognostic biomarker of severity, IP-10 (interferon-γ–induced protein 10), on the same chip. Because our test requires minimal user intervention and is read by a handheld detector, it can be globally deployed to combat COVID-19.
- Subjects :
- Coronavirus disease 2019 (COVID-19)
viruses
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Point-of-care testing
Concordance
Diseases and Disorders
Antibodies, Viral
Sensitivity and Specificity
Article
Serology
COVID-19 Serological Testing
03 medical and health sciences
Engineering
0302 clinical medicine
Medicine
Microneutralization Assay
Humans
030212 general & internal medicine
Seroconversion
skin and connective tissue diseases
Research Articles
030304 developmental biology
0303 health sciences
Multidisciplinary
biology
business.industry
SARS-CoV-2
fungi
SciAdv r-articles
COVID-19
Reproducibility of Results
Virology
respiratory tract diseases
body regions
Point-of-Care Testing
Spike Glycoprotein, Coronavirus
biology.protein
Antibody
business
Research Article
Subjects
Details
- ISSN :
- 23752548
- Volume :
- 7
- Issue :
- 26
- Database :
- OpenAIRE
- Journal :
- Science advances
- Accession number :
- edsair.doi.dedup.....73ddeb874b2e6c3492910efb52aaf668