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Intrauterine embryo transfer with canine embryos cryopreserved by the slow freezing and the Cryotop method
- Source :
- The Journal of Veterinary Medical Science
- Publication Year :
- 2016
-
Abstract
- Canine embryos (8-cell to blastocyst stages) frozen-thawed using the slow-freezing method with glycerol (four recipients) or dimethyl sulfoxide (three recipients) as a cryoprotectant and vitrified-warmed using the Cryotop method (five recipients) were surgically transferred into the unilateral uterine horn of recipient bitches. As a result, the morphology of embryos frozen-thawed using the slow-freezing method was judged to be normal, but no conception occurred in any of the recipient bitches. Two of the five bitches that received transferred embryos (morula to early blastocyst stages) vitrified-warmed using the Cryotop method became pregnant and produced normal pups (1/9 embryos, 11.1% and 1/6 embryos, 17.0%). It was concluded that the Cryotop method was more appropriate for canine embryo cryopreservation than the slow-freezing method, which is used for the cryopreservation of embryos of other mammalian species.
- Subjects :
- medicine.medical_specialty
animal structures
Cryoprotectant
Uterus
embryo
Biology
Cryopreservation
Time
Andrology
03 medical and health sciences
slow freezing method
0302 clinical medicine
Cryoprotective Agents
Dogs
Embryo cryopreservation
Pregnancy
medicine
Animals
Blastocyst
Gynecology
Cryotop method
030219 obstetrics & reproductive medicine
General Veterinary
Full Paper
0402 animal and dairy science
Embryo
Uterine horns
04 agricultural and veterinary sciences
Embryo Transfer
040201 dairy & animal science
Embryo transfer
medicine.anatomical_structure
embryonic structures
dog
Female
Theriogenology
Subjects
Details
- ISSN :
- 13477439
- Volume :
- 78
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- The Journal of veterinary medical science
- Accession number :
- edsair.doi.dedup.....73d5e359eb22f57019d535ffe352235b