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Synovial Fluid Cell Proteomic Analysis Identifies Upregulation of Alpha-Taxilin Proteins in Rheumatoid Arthritis: A Potential Prognostic Marker
- Source :
- Journal of Immunology Research, Vol 2020 (2020), Journal of Immunology Research
- Publication Year :
- 2020
- Publisher :
- Hindawi Limited, 2020.
-
Abstract
- Rheumatoid arthritis (RA) is a chronic autoimmune inflammatory disease affecting the joints and surrounding tissue. Identification of novel proteins associated with the progression of a disease is a prerequisite for understanding the pathogenesis of RA. The present study was undertaken to identify the potential biomarkers from a less explored biological sample such as synovial fluid (SF) cells which is specific for RA and to analyze their functional aspects using proteomic approach. Two-dimensional gel electrophoresis (2-DE) was performed using synovial fluid cells of RA and osteoarthritis (OA) patients, and 7 differentially expressed proteins were identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS/MS). Αlpha-Taxilin (α-Taxilin) has been found as one of the novel, significantly up regulated protein in RA. It has been validated in the synovium, synovial fluid (SF), SF cells, and plasma samples by Western blot, enzyme-linked immunosorbent assay (ELISA), fluorescence-activated cell sorting (FACS), immunohistochemistry (IHC), and real-time PCR. The identification of autoantibody against α-Taxilin and in silico studies has further helped us to understand its involvement in disease mechanism. The present study will therefore provide knowledge towards the etiology of RA that pave the way for suitable prognostic marker identification along with other clinical parameters.
- Subjects :
- Male
Proteomics
Article Subject
Immunology
Cell
Vesicular Transport Proteins
Arthritis, Rheumatoid
Pathogenesis
03 medical and health sciences
0302 clinical medicine
Western blot
Osteoarthritis
Synovial Fluid
medicine
Humans
Immunology and Allergy
Synovial fluid
Cells, Cultured
Autoantibodies
030304 developmental biology
030203 arthritis & rheumatology
0303 health sciences
medicine.diagnostic_test
business.industry
Autoantibody
General Medicine
Middle Aged
RC581-607
Cell sorting
Prognosis
medicine.disease
medicine.anatomical_structure
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Rheumatoid arthritis
Cancer research
Immunohistochemistry
Female
Immunologic diseases. Allergy
business
Biomarkers
Research Article
Subjects
Details
- ISSN :
- 23147156 and 23148861
- Volume :
- 2020
- Database :
- OpenAIRE
- Journal :
- Journal of Immunology Research
- Accession number :
- edsair.doi.dedup.....73bc7a3a012a33da3b19ac5e61ac183a