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Preparation of anionic poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) copolymeric nanoparticles as basic protein antigen carrier
- Source :
- Growth Factors. 25:202-208
- Publication Year :
- 2007
- Publisher :
- Informa UK Limited, 2007.
-
Abstract
- It is an useful method for polymeric nanoparticles to load protein by electrostatic method to improve the immunogenicity of protein antigen. In this article, anionic poly(e-caprolactone)-poly(ethylene glycol)-poly(e-caprolactone) (PCEC) nanoparticles were prepared by modified emulsion solvent evaporation method, and human basic fibroblast growth factor (bFGF), as a model protein, was absorbed onto its surface due to electrostatic interaction. The prepared anionic PCEC nanoparticles, with mean diameter of 136.9 nm, had zeta potential of − 33.14 mV. The surface charge and particle size of bFGF/nanoparticles complex increased with increase of bFGF/nanoparticles mass ratio. The encapsulated bFGF could be released slowly from bFGF/nanoparticle complexes. The animal experiment indicated that the humoral immunity induced by bFGF/PCEC complex was improved greatly than that created by naked bFGF. Otherwise, the cytotoxicity of anionic PCEC nanoparticles was also evaluated by 293 cell viability. The prepared anioni...
- Subjects :
- Anions
Polyesters
Static Electricity
Clinical Biochemistry
Basic fibroblast growth factor
Nanoparticle
Cell Line
Polyethylene Glycols
Mice
chemistry.chemical_compound
Endocrinology
Zeta potential
Animals
Humans
Surface charge
Antigens
Particle Size
Cytotoxicity
Drug Carriers
Chemistry
Cell Biology
Mice, Inbred C57BL
Antibody Formation
Nanoparticles
Female
Fibroblast Growth Factor 2
Particle size
Ethylene glycol
Caprolactone
Nuclear chemistry
Subjects
Details
- ISSN :
- 10292292 and 08977194
- Volume :
- 25
- Database :
- OpenAIRE
- Journal :
- Growth Factors
- Accession number :
- edsair.doi.dedup.....73a93d09067ad98a17da57d4bcb4d8c1