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Sensitive, Robust, and Cost-Effective Approach for Tyrosine Phosphoproteome Analysis
- Source :
- Analytical chemistry. 89(17)
- Publication Year :
- 2017
-
Abstract
- Albeit much less abundant than Ser/Thr phosphorylation (pSer/pThr), Tyr phosphorylation (pTyr) is considered as a hallmark in cellular signal transduction. However, its analysis at the proteome level remains challenging. The conventional immunopurification (IP) approach using antibodies specific to pTyr sites is known to have low sensitivity, poor reproducibility and high cost. Our recent study indicated that SH2 domain-derived pTyr-superbinder is a good replacement of pTyr antibody for the specific enrichment of pTyr peptides for phosphoproteomics analysis. In this study, we presented an efficient SH2 superbinder based workflow for the sensitive analysis of tyrosine phosphoproteome. This new method can identify 41% more pTyr peptides than the previous method. Its excellent performance was demonstrated by the analysis of a variety of different samples. For the highly tyrosine phosphorylated sample, for example, pervanadate-treated Jurkat T cells, it identified over 1800 high confident pTyr sites from only 2 mg of proteins. For the unstimulated Jurkat cells, where the pTyr events rarely occurred, it identified 343 high confident pTyr sites from 5 mg of proteins, which was 31% more than that obtained by the antibody-based method. For the heterogeneous sample of tissue, it identified 197 high confident pTyr sites from 5 mg protein digest of mouse skeletal muscle. In general, it is a sensitive, robust and cost-effective approach and would have wide applications in the study of the regulatory role of tyrosine phosphorylation in diverse physiological and pathological processes.
- Subjects :
- 0301 basic medicine
Proteomics
Proteome
Jurkat cells
Sensitivity and Specificity
Mass Spectrometry
Analytical Chemistry
03 medical and health sciences
Jurkat Cells
Humans
Amino Acid Sequence
Tyrosine
Phosphorylation
Peptide sequence
030102 biochemistry & molecular biology
Chemistry
Phosphoproteomics
Reproducibility of Results
Cellular signal transduction
030104 developmental biology
Biochemistry
Signal transduction
Peptides
Subjects
Details
- ISSN :
- 15206882
- Volume :
- 89
- Issue :
- 17
- Database :
- OpenAIRE
- Journal :
- Analytical chemistry
- Accession number :
- edsair.doi.dedup.....73a3346ab05247879c800ea27b8789cb