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Casein synthesis is independently and additively related to individual essential amino acid supply
- Source :
- Journal of Dairy Science. 97:2998-3005
- Publication Year :
- 2014
- Publisher :
- American Dairy Science Association, 2014.
-
Abstract
- Specific AA affect rates of milk protein synthesis in the mammary glands of lactating cows. The objective of this study was to quantify the rate of αS1-casein synthesis in response to Ile, Leu, Met, and Thr supplementation, and to test the single-limiting AA theory for milk protein synthesis by exploring interactions among these AA. Effects of Ile, Leu, Met, and Thr were studied in vitro with a composite design containing a central point repeated 4 times, with 2 axial points per AA and a complete 2(4) factorial. Other AA were at the concentration in Dulbecco's modified Eagle medium/F12 medium (DMEM). The experiment was replicated with mammary tissue from 5 lactating cows. Mammary tissue slices (0.12 ± 0.02 g) were incubated for 4h at 37°C in 5 mL of treatment medium containing (2)H5-Phe. Caseins were precipitated from cell homogenate supernatants. Enrichment with (2)H5-Phe of the N[34]LLRFFVAPFPE αS1 peptide was determined by matrix-assisted laser desorption/ionization-tandem time-of-flight (MALDI-TOF-TOF), which was used to determine enrichment of Phe in the transfer (t)RNA pool and αS1-casein fractional synthesis rates (CFSR). Data were analyzed with a polynomial mixed model containing linear, quadratic, and 2-factor interactions for Ile, Leu, Met, and Thr, and cow and residual as random factors. Interactions were not significant at P
- Subjects :
- medicine.medical_specialty
Peptide
Mammary Glands, Animal
Internal medicine
Casein
Genetics
Protein biosynthesis
medicine
Animals
Lactation
Amino Acids
Essential amino acid
chemistry.chemical_classification
Milk protein
TOR Serine-Threonine Kinases
Caseins
RNA
Mammary tissue
Milk Proteins
In vitro
Milk
Endocrinology
Gene Expression Regulation
chemistry
Cattle
Female
Animal Science and Zoology
Amino Acids, Essential
Signal Transduction
Food Science
Subjects
Details
- ISSN :
- 00220302
- Volume :
- 97
- Database :
- OpenAIRE
- Journal :
- Journal of Dairy Science
- Accession number :
- edsair.doi.dedup.....73a2fae041bd396033586dc6f67d826a