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Sirolimus-eluting stents: Opposite in vitro effects on the clonogenic cell potential on a long-term exposure
- Source :
- Oncotarget
- Publication Year :
- 2020
-
Abstract
- We evaluated the long-term effects of sirolimus on three different cell in vitro models, cultured in physiological conditions mimicking sirolimus-eluted stent, in order to clarify the effectiveness of sirolimus in blocking cell proliferation and survival. Three cells lines (WPMY-1 myofibroblasts, HT-29 colorectal adenocarcinoma, and U2OS osteosarcoma) were selected and growth in 10 ml of Minimum Essential Medium for 5 weeks with serial dilutions of sirolimus. The number of colonies and the number of cells per colony were counted. As main result, the number of WPMY-1 surviving colonies increased in a dose-dependent manner when treated with sirolimus (p = 0.0011), while the number of U2OS colonies progressively decreased (p = 0.0011). The clonal capacity of HT-29 was not modified by the exposure to sirolimus (p = 0.6679). In conclusion sirolimus showed the well-known cytostatic effect, but with an effect on clonogenic potential different among the different cell types. In the practice, the plaque typology and composition may influence the response to sirolimus and thus the effectiveness of eluted stent.
- Subjects :
- Cell type
medicine.medical_treatment
Cell
Andrology
medicine
Sirolimu
cardiovascular diseases
Clonogenic assay
Cell proliferation
Cell growth
Chemistry
Stent
medicine.disease
equipment and supplies
In vitro
medicine.anatomical_structure
sirolimus
surgical procedures, operative
Oncology
Sirolimus
Atherosclerosi
cardiovascular system
Osteosarcoma
Stents
atherosclerosis
medicine.drug
Research Paper
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Oncotarget
- Accession number :
- edsair.doi.dedup.....7383d0490a59497ab646e93e6fa7e3dd