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High-performance liquid chromatographic determination of N-nitroso-N-alkylureas by pre-column fluorescence derivatization and application to blood analysis

Authors :
Misa Kodama
Shoji Takitani
Megumi M. Ohashi
Akira Sano
Source :
Journal of chromatography. 621(2)
Publication Year :
1993

Abstract

A method for the derivatization and separation of N-nitroso-N-alkylureas [alkyl = methyl (NMU), ethyl (NEU), and n-butyl (NBU)] has been developed. Fluorescent derivatives were formed with sodium sulphide, taurine and o-phthalaldehyde and separated by reversed-phase high-performance liquid chromatography. The limits of detection of standard NMU, NEU and NBU were 0.25, 0.8 and 1.5 pmol/200 microliters, respectively. The method was applied to the determination of NMU in blood after extraction with acetonitrile in the presence of calcium chloride. NBU was used as the internal standard. The recovery of NMU from blood was ca. 95%, and the limit of detection was 10 pmol/400 microliters blood. NMU levels in rabbit blood following a single oral administration were also measured.

Details

Volume :
621
Issue :
2
Database :
OpenAIRE
Journal :
Journal of chromatography
Accession number :
edsair.doi.dedup.....7383635c53b44868a08c768aec139ef7