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High-performance liquid chromatographic determination of N-nitroso-N-alkylureas by pre-column fluorescence derivatization and application to blood analysis
- Source :
- Journal of chromatography. 621(2)
- Publication Year :
- 1993
-
Abstract
- A method for the derivatization and separation of N-nitroso-N-alkylureas [alkyl = methyl (NMU), ethyl (NEU), and n-butyl (NBU)] has been developed. Fluorescent derivatives were formed with sodium sulphide, taurine and o-phthalaldehyde and separated by reversed-phase high-performance liquid chromatography. The limits of detection of standard NMU, NEU and NBU were 0.25, 0.8 and 1.5 pmol/200 microliters, respectively. The method was applied to the determination of NMU in blood after extraction with acetonitrile in the presence of calcium chloride. NBU was used as the internal standard. The recovery of NMU from blood was ca. 95%, and the limit of detection was 10 pmol/400 microliters blood. NMU levels in rabbit blood following a single oral administration were also measured.
- Subjects :
- Acetonitriles
Taurine
Sulfides
Fluorescence
Nitrosourea Compounds
chemistry.chemical_compound
Calcium Chloride
Animals
Humans
Derivatization
Acetonitrile
Alkyl
Chromatography, High Pressure Liquid
Phthalaldehyde
chemistry.chemical_classification
Detection limit
Chromatography
Extraction (chemistry)
Methylnitrosourea
General Chemistry
Nitroso
chemistry
Ethylnitrosourea
Rabbits
o-Phthalaldehyde
Subjects
Details
- Volume :
- 621
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Journal of chromatography
- Accession number :
- edsair.doi.dedup.....7383635c53b44868a08c768aec139ef7