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Long-Term Expression of Erythropoietin from Myoblasts Immobilized in Biocompatible and Neovascularized Microcapsules
- Source :
- Molecular Therapy. 12:283-289
- Publication Year :
- 2005
- Publisher :
- Elsevier BV, 2005.
-
Abstract
- The present paper investigates the long-term functionality of an ex vivo gene therapy approach based on cell microencapsulation for the continuous delivery of erythropoietin (EPO) without implementation of immunosuppressive protocols. Polymer microcapsules (0.5 ml) loaded with EPO-secreting C(2)C(12) myoblasts and releasing 15,490 +/- 600 IU EPO/24 h were implanted in the peritoneum and subcutaneous tissue of syngeneic and allogeneic mice. High and constant hematocrit levels were maintained for more than 100 days in all implanted mice. Capsules retrieved from the peritoneum were free-floating or forming small capsule clusters, and we detected only a weak fibroblast outgrowth in capsules adhered to organs, whereas capsules explanted from the subcutaneous region appeared altogether as a richly vascularized structure with no signs of major host reaction. Interestingly, the functionality of capsules implanted in the allogeneic mice persisted until day 210 after implantation. These results highlight the feasibility of cell encapsulation technology for the long-term delivery of EPO independent of the method of administration and the mouse strain.
- Subjects :
- Capsules
Pharmacology
Cell Line
Myoblasts
Cell therapy
Mice
Coated Materials, Biocompatible
Peritoneum
Drug Discovery
Genetics
medicine
Animals
Cell encapsulation
Fibroblast
Erythropoietin
Molecular Biology
Mice, Inbred BALB C
Mice, Inbred C3H
Chemistry
Capsule
medicine.anatomical_structure
Cell culture
Immunology
Molecular Medicine
medicine.drug
Subcutaneous tissue
Subjects
Details
- ISSN :
- 15250016
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Molecular Therapy
- Accession number :
- edsair.doi.dedup.....737adecc18f9bde30ec505ce31f20df7