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Virological Surveillance of Dengue in Saint Martin and Saint Barthélemy, French West Indies, Using Blood Samples on Filter Paper
- Source :
- American Journal of Tropical Medicine and Hygiene, American Journal of Tropical Medicine and Hygiene, American Society of Tropical Medicine and Hygiene, 2012, 86 (1), pp.159-65. ⟨10.4269/ajtmh.2012.11-0475⟩
- Publication Year :
- 2012
- Publisher :
- American Society of Tropical Medicine and Hygiene, 2012.
-
Abstract
- International audience; To strengthen active dengue surveillance in Saint Martin and Saint Barthélemy, two French Caribbean islands, we evaluated the epidemiological usefulness of collecting blood samples from NS1-positive dengue patients on filter paper to identify the dengue serotypes circulating in these regions during a 27-month period. This approach allowed dengue serotypes to be identified by reverse transcriptase-polymerase chain reaction in 90.1% of the total set of 666 samples analyzed and, in 95.5% of the samples collected during the acute phase of the disease. This prospective virological surveillance using blood samples absorbed onto filter paper, which were stored at 4°C and shipped at ambient temperature to a specialized laboratory for analysis, allowed us to avoid the logistic and financial costs associated with shipping frozen venous blood samples. This surveillance system offers a low-cost alternative for reinforcing dengue prevention in areas where specialized laboratories do not exist, notably by facilitating the early detection of potentially new dengue serotypes.
- Subjects :
- Male
MESH: Blood Specimen Collection
MESH: Dengue
Dengue virus
MESH: Dengue Virus
medicine.disease_cause
Dengue fever
Dengue
0302 clinical medicine
MESH: Reverse Transcriptase Polymerase Chain Reaction
Prospective Studies
030212 general & internal medicine
West indies
Blood Specimen Collection
Caribbean island
Reverse Transcriptase Polymerase Chain Reaction
Articles
Saint Barthelemy
3. Good health
Infectious Diseases
Population Surveillance
Female
MESH: Paper
Paper
Financial costs
MESH: West Indies
West Indies
030231 tropical medicine
Early detection
Sensitivity and Specificity
MESH: Population Surveillance
03 medical and health sciences
Virology
Environmental health
medicine
Humans
Serotyping
MESH: Humans
business.industry
MESH: Serotyping
Dengue Virus
medicine.disease
MESH: Sensitivity and Specificity
MESH: Male
MESH: Prospective Studies
MESH: Filtration
[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie
Parasitology
business
MESH: Female
Filtration
Subjects
Details
- ISSN :
- 14761645 and 00029637
- Volume :
- 86
- Database :
- OpenAIRE
- Journal :
- The American Journal of Tropical Medicine and Hygiene
- Accession number :
- edsair.doi.dedup.....736e9caffb8d54aff8432722ab512978